A genetic map of the W incompatibility group plasmid pSa has been prepared through the construction of deletion derivatives of pSa and the cloning of various fragments of pSa in pBR322. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to chloramphenicol...
Bovine serum ■ 纯度 1. 200μg 的本制品和1μg 的λ DNA-Hind III 分解物在37℃、pH7.5 的条件下反应16小时, DNA片段的电泳图像不起变化。 2. 200μg 的本制品和1μg 的pBR322 超螺旋DNA在37℃、pH7.5 的条件下反应4小时,不发生DNA的解螺旋现象。
27、 from phage lambda, and T1 from the rrnB operon of E. coli, to prevent read-through transcription. 6.ColE1 origin of replication from pBR322 7. beta- lactamase gene (bla) confers ampicillin resistance 1.T5 promoter and two lac operator. 2. Ribosomal binding site RBS. 3. 6xHis- ta...
BACsarebacterialartificialchromosomes,andarebasedonanaturallyoccurringlargebacterialplasmid,theFfactor.BACvectorscanaccommodateDNAinsertsupto300kb,stillfairlyrespectablewhenneedingtoclonelargegenesormapandsequencecomplexgenomes.BACshaveseveraladvantagesoverYACs,whichmeanstheyareusedmoreextensivelynow.Mostofthehumangenomehas...
(Mob site) and can be mobilized with high frequency from the donor strains. The mobilizable vectors are derived from the commonly usedE. colivectors pACYC184, pACYC177, and pBR325, and are unable to replicate in strains outside the enteric bacterial group. Therefore, they are widely ...
The nine oris were R6K [27], RK2 [28], pBBR1 [29], pRO1600/ColE1 [26], RFS1010 [26], p15A [30], pSC101 [31], pUC [32], and pBR322/ROP [33]. The P. pantotrophus cells were treated to be electrically competent and a constant volume of 100 µL of these electrocompetent...
MCS, multiple cloning site; T7P, T7 promoter; 6x His, hexahistidine-tag; T7-tag, 11-amino acid peptide encoded in the leader sequence of T7 bacteriophage gene 10; pBR322 ori, origin of DNA replication; KanR, kanamycin resistance marker; f1, phage-derived origin of ssDNA replication; ...
Aliquots of 1 jJ.1 added to 1 jJ.10.5 M NaOH, 1 M NaCI were spotted onto each of two nitrocellulose filters and filters were processed for hybridization'" with radiolabelled probes to pBR322 DNA and BPV-l DNA23• Filter-spotted DNAs which gave a signal with the BPV-1 DNA probe ...
Source/Description: A 2.7kb BamHI fragment from CYNZ22 isolated by zeta-globin oligonucleotide was subcloned into the BamHI site of pBR322. Polymorphism: Mspl identifies a more than ten allele VNTR polymorphism with bands between 0.5kb and 1.3kb. Taql, Rsal, BamHI, Pstl and Hindlll also...
and the selection of an appropriate cloning vector is a matter of choice. Examples of recombinant DNA vectors for cloning and host cells which they can transform include, but is not limited to, the bacteriophage λ (E. coli), pBR322 (E. coli), pACYC177 (E. coli), pKT230 (gram-negativ...