It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites for cloning or subcloning restriction fragments. pBR327 is identical to pBR322, except that it is missing all nucleotides between 1,427 and 2,516. These sequences are deleted to ...
It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites for cloning or subcloning restriction fragments. pBR327 is identical to pBR322, except that it is missing all nucleotides between 1,427 and 2,516. These sequences are deleted to ...
direction of transcription and include the start and stop codons.Origin of replication coordinates include the region from the -35 promoter sequence of the RNAII transcript to the RNA/DNA switch point. bla (Ap R ) gene coordinates include the signal sequence.(x) = enzyme not available from ...
The lab-grown plasmids, which are used as a vector contain an origin of replication, cloning site and selection marker Vector ElementDescription Origin of Replication (ORI) DNA sequence where initiation of replication starts Selectable MarkerFor selecting bacteria containing desired plasmid, e.g.antibio...
pBR322plasmid.Inaddition,wesuggestedthat theSeqAproteincouldmodulateperiodicallytheinitiationofreplicationofthepBR322 plasmid.Thelatercouldbefixedbyitsoriginsequence,onahemimethylatedstate,during theinitiationofthereplication. CrownCopyrightÓ2010PublishedbyElsevierInc.Allrightsreserved. 1.Introduction Escherichia...
The potential of the cruciform structure formation as an important factor influencing p53 sequence-specific binding to natural DNA targets[J]. Biochem Biophys Res Commun, 2010, 391(3):1409-1414. [31]Zannis-Hadjopoulos M, Sibani S, Price GB. Eucaryotic replication origin binding proteins[J]. ...
Origin of replication in episomal bovine papilloma virus type 1 DNA isolated from transformed cells. The origin of replication of bovine papilloma virus type 1 (BPV-1) has been determined by isolating replicative intermediates (RI) of BPV-transformed hamst... Waldeck,R?sl,Zentgraf - 《Embo Journ...
Because pBr322 and pUC19 both contain the same origin of replication, originally from pMBI (1), the bacterium does not distinguish between the two for purposes of replication. In addition, as the difference in size between the plasmids pUC19 and pBR322 is quite significant, with sizes of ...
prototype recombinant inthis series, pSV2-cat, consisted ofthebeta-lactamase geneandorigin ofreplication frompBR322coupled toasimian virus 40(SV40) early t... C Gorman - 《Molecular & Cellular Biology》 被引量: 1.2万发表: 1982年 Genetic transformation of mouse embryos by microinjection of purifi...
pbr322 it was the first artificial cloning vector created in the laboratory by francisco bolivar and raymond l.rodriguez. also, pbr322 is bigger in size compared to its puc counterparts. thus, they have a slower rate of replication. this e.coli cloning vector has the origin of replication (...