(1990) Sequences near the origin of replication of the DHFR locus of Chinese hamster ovary cells adopt left-handed Z-DNA and triplex structures. J. Biol. Chem. 265, 21789±21796.Blanchi, A., Wells, R. D., Heintz, N. H., Caddle, M. S. (1990) Sequence near the origin of ...
(including DNA helicases) to the origin of replication, leading to the start of replication. Although the well-definedDNA sequencesthat serve as replication origins inbudding yeasthave been defined, DNA sequences that specify replication origins in other eukaryotes have been more difficult to define...
Prolate or c2-like phages are a large homologous group of viruses that infect the bacterium Lactococcus lactis. In a collection of 122 prolate phages, three distinct, non-cross-hybridizing groups of origins of DNA replication were found. The nonconserved sequence was confined to the template for...
Chromosomal replication ininitiates at a particular sequence in the parental DNA called the origin of replication (C) and proceeds bidirectionally. Initiation requires the interaction of various factors at a given time within the cell cycle, including RNA polymerase. Recently, mini-chromosomes have ...
The gene A protein of bacteriophage phiX174 has been used in vitro to convert phiX RFI DNA into the relaxed RFII form by nicking the viral strand. The nucleotide sequence at the 3' end of the nick has been determined as -- T G C T C C C C C A A C T T Goh. This sequence ...
During our studies involving protein-DNA interactions, we constructed plasmid pSAM to fulfill two requirements: 1) to facilitate transfer of cloned sequences from widely used expression vector pET-28a(+), and 2) to provide a vector compatible with pBR322-derived plasmids for use in cells harborin...
网络复制起点序列 网络释义 1. 复制起点序列 即自主复制序列, 又叫复制起点序列(replication origin sequence)。是顺式作用元件的一种。 emuch.net|基于33个网页
With advances in synthetic biology and genome engineering comes a heightened awareness of potential misuse related to biosafety concerns. A recent study employed machine learning to identify the lab-of-origin of DNA sequences to help mitigate some of the
agents useful in the diagnosis, prognosis or treatment of disease, particularly fungal infections and neoproliferative disease. Disclosed methods for identifying a gene encoding a protein which directly or indirectly associates with a selected DNA sequence involve: transforming an expression library of ...
Like pUB110, both plasmids contained an open reading frame encoding a putative plasmid recombination enzyme (Pre protein), which was located downstream of the RSA-site. On the basis of sequence comparisons between pUB110, pMV158, pTB913, pT181, pE194, pNE131 and pT48 two distinct families ...