Sal I and Pst I restriction endonuclease-generated DNA fragments that specify an FII-type incompatibility function ( inc FII) of the low copy number antibiotic resistance plasmid R6-5 have been cloned in the high copy number pBR322 plasmid vector. A 1-kilobase DNA sequence that contains this ...
DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker. Specificity PCNA Applications IF, Live cell imaging Vector type Mammalian expression vector Reporter TagRFP Publications ...
Within each rep-group, plasmids were further clustered based on the type of replicase gene present and their mobility, respectively. We found that the clusters “inc” (incompatibility group) and “rep_cluster” (uncharacterized types) were the dominant groups in the gut plasmidome (Fig. 1a) ...
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An integrative plasmid is a type of plasmid that can be used to directly manipulate the yeast chromosome by replacing a genomic copy of a gene, inserting a mutation or epitope tag, or linking a chromosomal locus to a selectable plasmid marker, enabling subsequent genetic analysis in yeast cells...
Samples taken from the reaction mixtures were subjected to high-performance liquid chromatography (HPLC) analysis with an Atlantis dc18 column (4.6 by 250 mm; Waters Corp., Milford, MA) and a Varian ProStar HPLC system (Varian, Inc., Walnut Creek, CA) set at 280 nm in a diode array ...
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Synonyms Plasmid classification ; Plasmid family ; Plasmid grouping ; Replicon type Definition Plasmid incompatibility refers to the inability of two plasmids to coexist stably over a number of generations in the same bacterial cell line. Generally, closely related plasmids tend to be incompatible, whil...
plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. ...
The sample libraries for Illumina sequencing were prepared using the Nextera XT library kit (Illumina, Inc.) and sequencing was performed with paired-end reads on the Miseq system (Illumina, Inc.). Sequencing reads were trimmed to remove Illumina specific adaptors and low quality bases using ...