1.2 质粒多聚体形成机制：同源重组 1976年，J R Bedbrook等在Cell发表文章：《Recombination between bacterial plasmids leading to the formation of plasmid multimers》，他们发现pMB9质粒DNA在rec+大肠杆菌中呈现出各种不同的大小，更大形式的pMB9质粒DNA是最小的MB9质粒多聚体。这种质粒多聚体的形成是通过两...
内毒素,又称脂多糖或LPS,是大肠杆菌等革兰氏阴性菌细胞膜的组成部分,如大肠杆菌(参见"Bacterial cell wall")。内毒素在质粒裂解纯化过程中释放出来,并显著降低内毒素敏感细胞株的转染效率。此外,内毒素与DNA竞争“自由”转染试剂,影响转染实验中质粒DNA的摄取。内毒素也诱导巨噬细胞和B细胞等免疫细胞发生非特异性...
The procedure has been used successfully for isolation of the large (128 kb), very-low-copynumber (1-2 copies per cell) plasmid pHCG3 and its derivatives fromOligotropha carboxidovorans. Yield of plasmid DNA was typically 3-6 µg plasmid DNA from 200 ml culture. ...
美['plæzmɪd] 英['plæzmɪd] n.〔遗〕质粒;质体 网络细菌质粒;质粒载体;细菌质体 复数:plasmids 英汉 英英 网络释义 n. 1. 〔遗〕质粒 2. 质体
Plate 1.0x105 cells per well in 0.5 ml of complete growth medium. Cell density should be 50-80% confluent on the day of transfection. (Optional) The day of transfection, remove growth medium from cells and replace with 0.5 ml of complete...
质粒广泛用于基础和应用生物学。然而,质粒的设计和构建,特别是那些携带复杂遗传信息的质粒,仍然是进行复杂生物实验中最耗时、劳动密集和限速的步骤之一。作者构建了了名为 PlasmidMaker 的多功能、强大、自动化的端到端平台,该平台允许以高通量方式无错误地构建具有几乎任何序列的质粒。该平台包括使用基于 Pyrococcus furi...
(Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted ...
[3]Fang Y, Sun H, Zhai J, et al. Antitumor activity of NF-κB decoy oligodeoxynucleotides in a prostate cancer cell line[J]. Asian Pac J Cancer Prev, 2011, 12(10): 2721-6. [4]Li X, Martinez-Ferrer M, Botta V, et al. Epithelial Hic-5/ARA55 expression contributes to prostate ...
QIAGEN 1027298 AllStars Cell Death Contr.siRNA (5nmol)QIAGEN 9019341 LED, yellow (585), RG6QIAGEN 9234712 Board, level sensor, BR8000 v1.02QIAGEN 9020469 Peltier module, GSCQIAGEN 9234541 Pumphead click'n'go; HSPS; BR3/9604QIAGEN 9234462 Coupling, CPC, inline, male, 8mmQIAGEN 9234406 Valve...
Rb-GFP报告基因是翌圣生物自主研发的用于检测Retinoblastoma protein转录活性水平为目的的报告基因。Retinoblastoma protein (Rb)是一种肿瘤抑制蛋白,在多种癌细胞中是发生功能障碍的。Rb是通过抑制处于分裂期细胞的细胞周期进程阻碍细胞的过度增殖。 Rb-GFP报告基因主要用于检测细胞中cell cycle信号通路、药物研究以及基因过...