Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).
Spin column format miniprep plasmid isolation kits from bacteria available are GET™ Plasmid DNA Miniprep for up to 20µg DNA from 1-5ml culture and a 96-well plate format, GET™ Plasmid DNA 96-Well. Select the EZ Yeast™ Plasmid Prep kit for yeast plasmid purification. Alternatively,...
Analysis OptionsDNA Sequencing* Full-Service DNA Sequence Confirmation Restriction Digest and Agarose Gel Analysis* Sterility Testing *Includes one reaction per plasmid; added reactions available as an add-on. FEATURES & BENEFITS No plasmid purification kit needed – Submit starting material and GENEWIZ ...
regimen of these plasmid kits has been optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA resulting in plasmids that are suitable for sensitive applications including transfection, in vivo injections, gene editing, recombinant viral vector production, PCR and sequencing...
1a). All metagenomic sequencing data were quality controlled using KneadData (version: 0.7.6) with default parameters. Reconstruction of plasmid catalog The gut plasmid catalog was constructed using Plaspline (version: 1.4) (https://github.com/Wanli-HE/Plaspline.git)36. First, de novo metagenome...
Recently, developments in high-throughput metagenomic sequencing have made it possible to study the plasmid metagenome, known as the plasmidome, and some studies have used this approach to explore plasmid community in the gut environment29–31. However, our under- standing of plasmids is still ...
Interestingly, the plasmid sequencing showed that these four transposon mutations were all inserted into a fimbriae-associated gene cluster (fim-cluster). This fim-cluster is a hybrid segment spanning a 7,949bp sequence, with a terminal inverted repeat sequence and two coding regions. In summary,...
E. et al. Absolute quantification of translational regulation and burden using combined sequencing approaches. Mol. Syst. Biol. 15, e8719 (2019). Article PubMed PubMed Central CAS Google Scholar Ceroni, F., Algar, R., Stan, G. B. & Ellis, T. Quantifying cellular capacity identifies ...
Rychlik W, Rhoads RE: A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA. Nucleic Acids Res. 1989, 17: 8543-8551. Article CAS Google Scholar Aiyar A, Xiang Y, Leis J: Site-directed mutagenesis using overlap extension...
However, they were recently re-classified as Pantoea ananatis on the basis of nucleotide sequencing of 16S rRNA and so forth. Specifically, the gene of the present invention can be obtained from the Pantoea ananatis AJ13601 (FERM BP-7207) strain (EP1078989A). The Pantoea ananatis AJ13601 ...