一、质体构筑(Plasmid Construction) 19 二、细胞培养 (Cell Culture) 与基因转移感染 (Transfection) 20 三、免疫萤光染色法 (I… etds.lib.nchu.edu.tw|基于6个网页 3. 质体建构 2-2.质体建构(Plasmid construction) 13 2-3. 质体转殖 (Transformation) 18 2-4. ...
One or more donor DNA fragments encoding the protein of interest are combined with an acceptor plasmid, a first DNA linker, and a second DNA linker in a Saccharomyces cerevisiae host cell whereby the donor DNA fragment is joined to the acceptor plasmid by homologous recombination of the donor ...
Plasmid Construction Construction of plasmids involves the usage of enzymes referred to as restriction enzymes. These enzymes are used to cut the DNA at specific sites known as restriction sites. The resulting fragments are then joined together using another enzyme referred to as DNA ligase. ...
质粒构建,Plasmid construction 1)Plasmid construction质粒构建 英文短句/例句 1.Construction and Expression in E.coli of the Recombinant Plasimid pET30a-EgA31-Eg95 of Echinococcus Granulosus and Its Serological Evaluation细粒棘球绦虫pET30a-EgA31-Eg95重组质粒构建及鉴定 2.Construction and identification of...
4) plasmid construction and expression 质粒构建与表达 例句>> 5) plasmid pMC71A LEU pMC71A-LEU质粒构建6) PTHrP chondrosarcoma siRNA(PTHrp)质粒构建补充资料:PUC18质粒DNA CAS: 9007-49-2 中文名称: PUC18质粒脱氧核糖核酸;PUC18质粒DNA 英文名称: Deoxyribonucleic-acid;Calf thymus DNA;Desoxyribose ...
Elabscience®provides fast, high throughput, high quality, and personalized gene synthesis and plasmid construction services. It not only meets the needs of small amounts of plasmid for research experiments but also provides large-scale plasmids for industrial users in the biotech industry and pharmac...
Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-consuming, labor-intensive, and rate-limiting steps in perf
PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction 论文摘要 质粒广泛用于基础和应用生物学。然而,质粒的设计和构建,特别是那些携带复杂遗传信息的质粒,仍然是进行复杂生物实验中最耗时、劳动密集和限速的步骤之一。作者构建了了名为 PlasmidMaker 的多功能、强...
Plasmid construction|质粒构建 基本信息更多信息 中文名称:质粒构建 中文同义词:质粒构建 英文名称:Plasmid construction 英文同义词: CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览Plasmid construction国外供应商中国供应商 Plasmid construction推荐供应商 ...
coli DH5α66 was used during plasmid construction. All strains were routinely grown at 37 °C in Luria Bertani (LB) medium at 250 rpm shaking. For molecular cloning and documentation purpose, the plasmid carrying strains were grown either in LB supplemented with 25 µg kanamycin per ...