12,13,14,15. For example, high throughput synthesis of transcription activator-like effector nucleases (TALENs)13was achieved using automated construction of plasmids via Golden Gate method. In addition, a web-
A totally polymerase chain reaction (PCR)-based protocol for construction of plasmids for production of recombinant macropodid herpesvirus 1 (MaHV-1) is described. This protocol greatly simplifies traditional methods that use restriction enzyme-based cloning or a combination of restriction enzyme cloning...
2.3 Construction of pUBER plasmid The plasmid pUBER (Addgene plasmid 80716) was generated as previously described (Mueller et al., 2020). Briefly, two synthetic double-stranded gene blocks were inserted into pSuperCos1 plasmid (van Loenhout, De Grunt, & Dekker, 2012). The key gene block ins...
From custom vector design to high-throughput protein expression, we have a solution to meet your needs. Plasmid Services GeneArt® Plasmid Services include flexible offerings covering construction of plasmid vectors customized for your needs, and subc...
By consistently providing DNA plasmid under quality standards consistent with GLP conditions for our in-house downstream services, such as transient protein expression, stable cell line construction, and antibody drug development, GenScript has a level of quality that other suppliers cannot achieve for ...
For the construction of the plasmid pIND, the nptII gene (primer: nptII_for/rev) was sub-cloned into the vector pBAD3068 that contains the ara-operon using restriction enzyme cloning (cutsites: EcoRI and HindIII). Thereafter, the ara-operon containing the nptII gene was amplified from p...
Tsuji, Takemasa; et al. Rapid construction of antitumor T-cell receptor vectors from frozen tumors for engineered T-cell therapy. Cancer Immunology Research 6 5 594-604 2018. Shi, Xiaojing; et al. Genetically engineered cell-derived nanoparticles for targeted breast cancer immunotherapy. Molecular ...
Looking to enhance performance and manufacturing from your DNA backbone, without the regulatory concerns? The small Nanoplasmid backbone fulfills all the requirements. The Nanoplasmid Vector System (<500 bp backbone) provides advantages over existing legacy plasmid DNA backbones and minicircles: ...
Conjugative plasmids promote the dissemination and evolution of antimicrobial resistance in bacterial pathogens. However, plasmid acquisition can produce physiological alterations in the bacterial host, leading to potential fitness costs that determine the clinical success of bacteria-plasmid associations. In th...
transformation into the final host. Construction of expression vectors for use in a predetermined species of host cell is routine in the art, and many such vectors and vector components can be purchased from commercial suppliers. The methods of the present invention can be used to produce ...