The overall end-to-end pipeline for PlasmidMaker is shown in Fig.1. There are four major steps involved in the automated workflow for plasmid construction: (1) In the Design part, users can design plasmids by a
Plasmid Services GeneArt® Plasmid Services include flexible offerings covering construction of plasmid vectors customized for your needs, and subcloning of your sequence into any vector, including the Gateway® vector system. Concentrate on your resea...
The invention provides an shRNA expression plasmid inhibiting CYP3A4. Three shRNA template sequences which meet requirements are obtained by eliminating a sequence with homology with other genes in a genome by Blast software according to an interference fragment designing principle and a CYP3A4 ...
21.1.1 Strain construction We use a strategy similar to that described previously (Brickner et al., 2010) to create yeast strains that allow the determination of the subnuclear localization of genomic loci. However, whereas the strains described previously are only useful for immunofluorescence, the...
www.nature.com/scientificreports OPEN received: 26 January 2016 accepted: 04 May 2016 Published: 26 May 2016 Construction of plasmid-free Escherichia coli for the production of arabitol-free xylitol from corncob hemicellulosic hydrolysate Buli Su, Zhe Zhang, Mianbin Wu, Jianping Lin & ...
Construction of potential plasmid transfer network Based on the number and identity of plasmids associated with different hosts, we constructed a network of putative horizontal plasmid transmission events. Specifically, a plasmid transfer event was presumed to have occurred if plasmids with the same conti...
DNAs are high molecular-weight molecules with double strands that contain transgenes for specific proteins. In therapeutics, DNA inhibits the generation of specific protein through the release of transgenes into cells. The design and construction of plasmids with transgene(s) of interest is an impor...
Abstract Conjugative plasmids promote the dissemination and evolution of antimicrobial resistance in bacterial pathogens. However, plasmid acquisition can produce physiological alterations in the bacterial host, leading to potential fitness costs that determine the clinical success of bacteria-plasmid associations...
Construction of gene families Protein-coding genes were extracted from all complete assemblies and clustered into protein families based on amino acid sequence similarity. For that, reciprocal best hits (RBHs) among all contigs were identified using the easy-RBH module of MMSeqs [31] v14.7 with a...
Under construction Output Since v1.6, the more relevant output is located in GROUP/SAMPLE folder: SAMPLE_final_results.html(.tab) id: Name of the accession number of reference length: length of the reference sequence species: species of the reference sequence ...