Concentration Nanodrop ✓ ✓ ✓ Identity Plasmid size Restriction digestion ✓ ✓ ✓ Insert sequence Sanger sequencing Optional Optional Optional Full-length plasmid sequence Sanger sequencing Optional Optional Optional NGS Optional Optional Optional Purity OD260/280 Nanodrop ✓ ✓ ✓ Supercoile...
3) you can do: 2.5ml of chloramphenicol (34mg/ml dissolved in ethanol), the final concentration of 170 mu g/ml. such as pBR322 in host bacteria to medium copy plasmid Ganhang Lou complex is necessary, through the amplification. These plasmids can be copied to a very high copy number ...
GenScript's research-grade plasmid DNA can help your on standard preps for cloning, mutagenesis and transformation, from microgram- to gram-scale with reliable quality and fast turnaround time to accommodate tight deadlines, $30 for 100 µg.
Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free plasmid purification kits from Supplier Q and Supplier MN.Plasmid DNA (pGL3®) was isolated from 150 ml of JM109E. coliculture grown overnight following the manufacturer’s suggested...
Concentration0.5 μg/μL Sample TypeDNA Final Product TypeDNA For Use With (Application)Cloning Product TypePlasmid DNA Quantity100 μg VectorpBR322 Unit Size100 µg 浏览此商品的顾客同时也查看了 pcDNA™3.1 (+) 哺乳动物表达载体 Catalog number: V79020 11,566.00 / 20 µgAdd to cart pUC19...
AntibioticEffectWorking ConcentrationStock Solution Ampicillin (Amp) Kills replicating cells 50-100 μg/mL. 50 mg/mL in H2O Chloramphenicol (Cm) Bacteriostatic 20-170 μg/mL 34 mg/mL in ethanol Kanamycin (Kan) Bactericidal 30 μg/mL 50 mg/mL in H2O Streptomycin (Sm) Bactericidal 30 μg/mL...
5、The relationship of biomass growth and concentration of plasmid was investigated.研究了包括菌体生长和质粒含量等的关系。 6、A Rapid Method for Mini-preparation ot Plasmid DNA介绍质粒DNA快速微量制备法 7、Results show that carbon source, nitrogen source affected plasmid DNA yield significantly.实验结...
(6) Measure the DNA concentration. step 4 ligating and transforming into bacteria (1)连接 4ul oligo from step2 20ng (or 1ul) PLKO step3 2ul 10x NEB T4 DNA ligase buffer 1ul NEB T4 DNA ligase to 20ul ddH2O incubate at 16℃ for 4-20h ...
The construction of a vector for the expression of fusion proteins requires the subcloning of the cDNA of interest into a vector’s backbone, at a site next to one or more pre-existing in-frame modules that provide the functions to be monitored. Hence, the design of the preassembled backbo...
10 μg pUB110 plasmid DNA was linearized with approximately 10 units of the restriction endonuclease Xba I in a buffer system of final concentration 50 l mM of Tris-HCl, 5 mM of MgCl2. Restriction was carried out at 37° C. for 60 minutes. Similarly 10 μg pUB109 was linearized with ...