Timmis, K.N., Cohen, S.N., Cabello, F.: DNA cloning and the analysis of plasmid structure and function. Prog. molec. subcell. Biol. 6 (1978d)Timmis, K.N., Cohen, S.N., Cabello, F.C. 1978. DNA cloning and the analysis of plasmid structure and function. Progress in Molecular...
For the screening step, a very large number of sequences that are typically generated in the design phase is then filtered first computationally (in silico) and second experimentally (in vitro), often resulting in multiple design-and-screen iterations2. Hence, for the screening of proteins ...
performed cloning experiments. K.S. provided all transgenic mice. O.G.S. contributed to data analysis and preparation of the manuscript. J.S.L. performed electrophysiology experiments and analysis related to NMDA receptors expressed in HEK293T cells. K.B.H. performed analysis of data for NMDA...
The qPCR data were analyzed using ANOVA followed by Tukey’s multiple comparison test. 2.4. Functional Studies of RdPrip through RNA Interference The dsRdPrip RNA was synthesized using the MEGAscript RNAi Kit (Thermo), following the manufacturer’s instructions. The plasmid containing the cDNA of ...
NRSE (pNRSE450) or mutant NRSE (pmNRSE450) were cloned into the multiple cloning site of the pGL3control luciferase reporter vector. (B) Total RNA was extracted from transfected cells and transcripts were quantified by RT-qPCR. (C) The promoter activity of each construct is expressed as ...
Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions. Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a...
Mutagenesis, according to the procedure described by Kunkel (20, 21), was first used to create aMluI cloning site in the cDNA encoding the stem region of the enzyme at amino acid 40, which is the position of the native N terminus for the secreted form of the enzyme. This construct, ...
Monocytes and T cells are two major subpopulations of peripheral blood mononuclear cells (PBMC) and play an essential role in the innate and adaptive immune systems. Different members of the galectin family show multiple and distinct regulatory effects on different cell types. Previous studies have ...
Klebsiella pneumoniae plays a major role in causing nosocomial infection in immunocompromised patients. Medical inflictions by the pathogen can range from respiratory and urinary tract infections, septicemia and primarily, pneumonia. As more K. pneumonia
Plasmid pNeb-DT-GATA6 was generated by cloning a 12.2 kb EcoRV/PacI genomic fragment containing Gata6 exons 1 and 2 into the PacI/HincII site of a vector, pNEB193-DT, which contained a diphtheria toxin (DT) expression cassette to enrich against random integration of the targeting plasmid ...