The problem that analysis on a specific gene function cannot be smoothly carried out because the categories of enzyme digestion sites included in multiple cloning site (MCS) sequences of the expression carrier difficultly meet a specific research requirement is solved. The artificially designed and ...
The expression plasmids were generated using a combination of polymerase chain reaction (PCR), restriction enzyme digestion, and Gibson Assembly molecular cloning techniques. For Gibson Assembly reactions, vector DNA was digested with restriction enzymes cutting the multiple cloning site. All other DNA fr...
Insertional mutations were constructed by cloning a 750-bp sequence from the gene to be disrupted into the pKNOCK tet vector, followed by its introduction into the B. theta chromosome30. Growth of the WT and mutants was measured on glycans by mixing the autoclave-sterilised polysaccharides (0.5...
If you are interested in the problem we mentioned here, please refer to GitHub's HTTPS cloning errors article.Git InstallIf you have git installed (requires git v1.7.10+):clone this repo in the root of your user profile cd ~/ from anywhere then git clone https://github.com/nvm-sh/...
Cloning, nucleotide sequence, mutagenesis, and mapping of the Bacillus subtilis pbpD gene, which codes for penicillin-binding protein 4. The gene encoding penicillin-binding protein 4 (PBP 4) of Bacillus subtilis, pbpD, was cloned by two independent methods. PBP 4 was purified, and the amino...
The search feature is an important and useful function of Windows 11/10, and the index section may temporarily occupy a large number of system resources, thereby affecting the performance of Windows 11/10. To speed up Windows 11/10 and make it run better, you can try to disable search ind...
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Although the physiological functions of these enzymes are not yet fully understood, recent cloning and more in-depth characterization of acyl-CoA thioesterases has assisted in the discussion of functions for specific enzymes such as in the regulation and termination of fatty acid oxidation in ...
The site of transposon insertion in B67 was determined by “rescue cloning” according to the manufacturer's (Epicentre) protocol, and sequenced using primer R6KAN-2 RP-1. Sequencing analysis revealed an insertion in the XC_0714 (prc) gene (1534 bp downstream of the translation start site) ...
Molecular Cloning: a laboratory manual. 2001, New York: Cold Spring Harbor Laboratory Press, 1: 3 Google Scholar Tusher VG, Tibshirani R, Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA. 2001, 98: 5116-5121. 10.1073/pnas....