多克隆位点(multiple cloning site, MCS),是包含多个(一般大于20个)限制性酶切位点(restriction site)的一段很短的DNA序列。也称为多位点接头(polylinker),是基因工程中常用到的载体___的标准配置序列。MCS中,一个限制性酶切位点通常是唯一的,即它们在一个特定的载体质粒中只出现一次(百度百科)相关知识点...
网络多克隆位点区 网络释义 1. 多克隆位点区 ... 购得。 穿梭载体(shuttlevector)多克隆位点区(multiplecloningsite):由于不同基因的末端可以由不同的限制酶所产生, … www.docin.com|基于2个网页
68 2021-11 2 replicon复制子 61 2021-11 3 DNA libraries DNA文库 66 2021-11 4 multiple cloning sites多克隆位点 69 2021-11 5 restriction mapping限制性酶切图谱 63 2021-11 6 the PCR cycle聚合酶链式反应循环 82 2021-11 7 reporter genes报告基因 ...
Two types of multiple cloning site (MCS) were designed based on the MCS in the expression vector λgt11 Sfi-Not . In the first set of vectors a start Met codon was included in the same reading frame as in λ gt11 Sfi - Not to support expression of partial cDNA clones. Thus a cDNA...
(i) the replication origins of pUC and of the broad-host-range enterococcal plasmid pAMβ1; (ii) an erythromycin-resistance-encoding gene expressed in Gram− and Gram+ bacteria; (iii) the transfer origin of the IncP plasmid RK2; and (iv) the multiple cloning site and the lacZα ...
Cloning and Constructing of Eukaryotic Expression Vector of H-FABP Gene in Pig Heart fatty acid binding protein gene(H-FABP) is one of the most important candidate genes that affect pig's intramuscular fat content.In this research,the... QI Shun-Min,MA Kang,WU Ming-Ming,... - 《Chinese...
Plasmids expressing dXCas9-VPR were constructed by introducing mutations into the dCas9 region predicted to improve activity36 to generate pMM7-9-3 which also has a NotI linearization site used for cloning promoter and 5′UTR sequences. Plasmids expressing sgRNAs were generated by cloning annealed...
Staphylococcus pseudintermedius, a commensal and pathogen of dogs and occasionally of humans, expresses surface proteins potentially involved in host colonization and pathogenesis. Here, we describe the cloning and characterization of SpsD, a surface protein of S. pseudintermedius reported as interacting wi...
A first step in Cre-mediated site-specific DNA recombination is binding to the two 13 bp repeats of the 34 bp site loxP. Several nucleotides within loxP do... AW Rüfer,S Brian - 《Nucleic Acids Research》 被引量: 51发表: 2002年 Towards the directed evolution of virus-like particles der...
The vector consists of the plus origin of replication (Ori+) of the lactococcal plasmid pWV01, the sucrose genes of the lactic acid bacterium Pediococcus pentosaceus PPE1.0 as selectable marker, a multiple-cloning site, and a lactococcal DNA fragment of a well-characterized chromosomal region. ...