multiple cloning site sequence is inserted between a promoter of the rpsL gene and an open reading frame by a PCR overlapping and extending means firstly, and then a DNA fragment is cloned to a plasmid pBluescript KS with high copies, the multiple cloning site sequence of which is removed....
can be repeatedinmultiplethreads , to avoid duplication analysisorcloningusing performance loss. javakaiyuan.com javakaiyuan.com (2) 解析结果被表示成一个线程安全(不变类级)的指令树,单个实例可以在多线程中任意重复使用,避免使用重复解析或克隆等性能损耗。
Plasmid vectors containing multiple cloning sites suitable for the rapid insertion of protein-coding sequences into poxviruses have been constructed. They are based on pUC plasmids and carry the thymidine kinase (TK) gene of vaccinia virus interrupted by a vaccinia virus promoter. Six unique restrict...
34 nt of theloxP2272 sequence, and a multiple cloning site (MCS) into intron 1 of theRosa26locus.bAfter microinjection of overlapping sgRNAs, Cas9 mRNA, and ssODNs, PCR-based ssODN-mediated KI efficiency
展开 关键词: Chromosomes, Bacterial Enterobacter cloacae DNA, Bacterial DNA Transposable Elements Blotting, Southern Cloning, Molecular Base Sequence Repetitive Sequences, Nucleic Acid Methylation Mutation DOI: http://dx.doi.org/ 被引量: 25 年份: 1991 收藏...
The availability of well-assembled genome sequences and reduced sequencing costs have enabled the resequencing of many additional accessions in several crops, thus facilitating the rapid discovery and development of simple sequence repeat (SSR) markers. Although the genome sequence of inbred spinach line...
Cloning, nucleotide sequence, mutagenesis, and mapping of the Bacillus subtilis pbpD gene, which codes for penicillin-binding protein 4. The gene encoding penicillin-binding protein 4 (PBP 4) of Bacillus subtilis, pbpD, was cloned by two independent methods. PBP 4 was purified, and the amino...
Contig Maps in Multiple Species Parallel Construction of Orthologous Sequence-Ready Cloneservice Email alerting click here top right corner of the article or ... J Idol,J Ryan,P Thomas,... 被引量: 0发表: 2002年 Cloning and developmental expression of a family of pleurocidin-like antimicrobial...
CoCl2artificially induces hypoxia29, which has allowed the characterization of the hypoxia response at the cellular, biochemical and molecular levels30. Moreover, we developed a computational tool, napSeeker, to determine the full-length sequences of napRNAs from total NAP-seq data (Supplementary Fig...
site mutants were constructed by overlapping PCR and using the nearest unique restriction sites present in pSP6-SFV4. Double and Quintuple Cp binding site mutants were constructed by restriction digest and subcloning from individual mutants. The 17-site mutant, Full PS mutant, and 17-site + ...