Plasmid vectors containing multiple cloning sites suitable for the rapid insertion of protein-coding sequences into poxviruses have been constructed. They are based on pUC plasmids and carry the thymidine kinase (TK) gene of vaccinia virus interrupted by a vaccinia virus promoter. Six unique restrict...
Plasmid vectors containing multiple cloning sites suitable for the rapid insertion of protein-coding sequences into poxviruses have been constructed. They are based on pUC plasmids and carry the thymidine kinase (TK) gene of vaccinia virus interrupted by a vaccinia virus promoter. Six unique restrict...
(an array of multiple restriction sites for cloning, also known asmultiple cloning site, MCS), splits thelacZgene. Thus, transformed cells containing the plasmid with inserted gene can be distinguished from cells containing the original (nonrecombinant) plasmid by the color of the colonies they ...
Vieira, JeffreyMessing, Joachim
Cloning of short hairpin RNAs for gene knockdown in mammalian cells The resulting PCR fragments are then cloned into the hairpin cloning site of the vector pSM2, the clones are verified by sequencing, and the construct ... PJ Paddison,M Cleary,JM Silva,... - 《Nature Methods》 被引量: ...
Plasmid pPIDKTR2 was constructed using the pSB1K3 (Biobrick) vector as the backbone and inserting the Plac promoter, the N-terminal CI DNA binding domain gene mutation (CI(N,mut))), a multiple cloning site (MCS), a terminator and the triple detection sequence (Tri) according to the Bio...
To prepare minicircle DNA, desired transgenes are first cloned into a donor host plasmid (p5087). This plasmid was derived from the MN530A-1 (System Biosciences) by replacing the GFP Reporter sequence with a multiple cloning site using the restriction enzymesXmaIandStuIand duplexed donor oligo...
One repetitive sequence was identical to the nucleotide sequence of IS10-right (IS10R), which is the active element in the plasmid-associated transposon Tn10. Unexpectedly, 15 copies of IS10R were found in the chromosomal DNA of E. cloacae MD36. One copy of the central region of Tn10 ...
Upon sequencing of our luciferase reporters, we unexpectedly identified a DRE core motif within the multiple cloning site (mcsDRE) of the pGL3 luciferase plasmid backbone in a subset of our reporters. Therefore, the objective of this study was to determine if the mcsDRE inadvertently influences ...
P. falciparumwas cultured in RPMI 1640 with Albumax® (Invitrogen), and schizonts were purified on a bed of 70% Percoll® as described previously [63]. About 10 μg of plasmid was ethanol precipitated and resuspended in 10 μL sterile buffer Tris-ethylenediaminetetraacetic acid (EDTA) (Qi...