Versatile: directly analyze normal, apoptotic, and dead cells by flow cytometry and fluorescence microscopy. Protocol Induce apoptosis by a desired method. A negative control should be prepared without inducing reagent. Harvest the cells, wash with cold PBS or culture medium and adjust the cell dens...
is a scalable microscopy system for Raspberry Pi. Here we include software and hardware documentation for building Mi-Pi. The hardware for each individual system costs ~$200, and each system is capable of continuous video capture and motion detection. We use this system to scale up behavior col...
Focus Scanner for Objectives and XY Stage with Piezo Drive for Inverted MicroscopesWith the P-725.xCDE1S focus scanner and the U-781 PILine XY stage system, PI completes its range of positioning systems for fluorescence microscopy applications. The dynamic focus scanner is offered as a complete ...
ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging. Development 142, 4168–4179 (2015). CAS PubMed PubMed Central Google Scholar Musielak, T. J., Schenkel, L., Kolb, M., Henschen, A. & Bayer, M. A simple and versatile cell wall staining protocol to ...
After 72 h, the cells were fixed with 4% (v/v) formaldehyde for 10 min on ice. They were then permeabilized and TUNEL labeled for DNA strand breaks using the in situ Cell Death Detection Kit (Roche, West Sussex, UK) according to the manufacturer's protocol. After counterstaining with ...
A method for confocal 4 - π - microscopy is characterized by the coherent illuminating a sample of two sides in each case by means of a lens with the illumination light, the at least one illumination waves length, by interference of the illumination light in the sample a standing illuminatin...
Viability/Cytotoxicity cell staining kit (Calcein AM, PI) Flow cytometry 1. Thaw reagents and prepare staining working solutions by adding 5 μL of component A (Calcein AM) and 30 μL of component B (PI) to 10 mL of PBS, and vortex to mix. 2. Wash the cells 2-3 times with 1X ...
The Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit with Alexa® Fluor 488 annexin V and PI for flow cytometry provides a rapid and convenient assay for apoptosis. The kit contains recombinant annexin V conjugated to one of our best and brightest fluorophores,the Alexa Fluor® 488 dye...
The invention relates to a confocal 4蟺 microscopy method which is characterized by coherently illuminating a sample from two sides by one objective each with illumination light which has at least one illumination wavelength whereby a stationary illumination wave having a main illumination maximum and ...
(CPE) was scored with light microscopy and 50% inhibitory concentration (IC50) was calculated using the spectrophotometric MTS/PES viability staining assay (Cell-Titer96 Aqueous One Solution Proliferation Assay kit; Promega, Leiden, The Netherlands). Absorbance was measured using the Versamax microplate...