PI staining FACS PI staining 实验目的：检测带有不同目的片段的相应质粒分别电转进HepG2后，细胞生长周期的变化情况（电转48 h后分析）实验材料：带有不同质粒的HepG2，PI/RNase Staining Buffer（BD, Cat No: 550825，贮存于4℃），-20℃ 75%乙醇，2% Triton X-100 实验步骤：1.收集细胞培养皿中的medium...
especially not after fixation. Keep in mind though, that fixation of your cells after staining with e.g. PI or 7AAD will partially permeabilize all your cells, so that PI or 7AAD can leak out of the
英文名称:Haematorylin Staining Solution 规格:100ml 苏木素是一种天然染料,是组胚,病理,泌尿,妇产科及各实验室中最常用的染色剂,主要用于细胞核染色。带正电荷的碱性染料苏木素与细胞核中带负电荷的酸性物质结合使细胞核染成蓝色。 操作步骤 1. 将样本涂于清洁的玻片上,制成薄涂片 ...
PI or 7-AAD can be included for cell cycle determination, although it is very difficult to obtain tight CVs. The protocol below is carried out in 96-well V-bottomed micro titer plates (MTP); with some modifications, it could be carried out in 12 x 75 mm plastic tubes. Phoenix Flow ...
all of which are part of the final staining panel. The FMO control allows determination of the cut-off between cells that are negative vs. positive for the CD3 marker and allow proper gate setting. The same approach is used to set the gates for the dead cells (PI) and the CD45 and ...
experiment of BCMA-CAR-T cells, both methods were compared head-to-head. In flow-cytometric analysis, the detection reagents used in this study could all detect BCMA-CAR-T cells at a similar level. The results of false-positive background staining differed as follows (standard deviation): ...
Titration experiments are easy to perform, and there are several papers that discuss this process1,2. This protocol has been optimized for 1×106cells in 50 µl final volume. All staining is performed on ice, in the dark, for 20 minutes. Identify the 1x concentration, which is the manu...
Green fluorescent protein–propidium iodide (GFP‐PI) based assay for flow cytometric measurement of bacterial viability Background Several staining protocols have been developed for flow cytometric analysis of bacterial viability. One promising method is dual staining with t... J Lehtinen,J Nuutila,...
QODG induces apoptosis in HUVECs as evidenced by annexin V/PI double staining and FACS analysis.Chen, Lin
number of dead and viable cells, the number of total cells from the spheroids/aggregates were counted using a Fuchs-Rosenthal cell counting chamber and multiplied with the percentage of viable or dead cells of the same spheroids/aggregates as determined from the annexin-V-fluos/PI staining ...