MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiol 9, 11. 2009.Metscher BD. MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal ...
微卫星技术(micro-satellite,-MS) 微卫星DNA又称短串联重复序列(short tandem repeats, STR) 、简单重复序列(simple sequence repeat, SSR),指DNA基因组中小于10个核苷酸的简单重复序列,广泛存在于真核基因组中,多数以2～6个碱基为核心单位、串联重复排列的序列。 1974年,S 2019-10-27 11:43 News WIKI 相关...
Staining protocol and other parameters for CMC plate assay were same as the laminarin plate assay. Full size image Figure 3 Superior resolution of optical fabric brightener (OFB) vs. congo red (CR) as a stain for the laminarin plate assay. Short Time Incubation (STI) samples (60%-90% ...
The protocol yielded acceptable assay statistics and demonstrated that iNs can now be produced and screened in high throughput settings. The assay performance might still be enhanced by opting for more expensive plate-coatings, multi-step antibody staining, and longer time points, but this pilot ...
Massive steatohepatitis with numerous hepatocyte bal- looning (arrow) and microvesicular steatosis (arrowhead) were present in all OLETF rats treated with ethanol, irrespective of number of days treated. (B) H&E staining of liver tissues from 10- and 33-wk-old untreated OLET rats and 30-wk-...
Therefore, in addition to obtaining increasingly higher resolution, technology improvements in the 2D gel community have been directed toward the image analysis of 2D gels and the relative quantitation of protein spots by their intensity of staining (3., 4., 5., 6.). Mass spectrometry has long...
Please observe all additional policies set by the local IACUC and ensure an IACUC-approved animal use protocol (or equivalent) is in place before proceeding with the following sections. Figure 3: Surgical instruments. (A) Surgical kit. Micro-drill (1), cutting wheel (2), absorbable suture (...
For the three antibodies whose epitopes were sensitive to methanol pretreatment (anti-PECAM, anti-cCasp9, and one of the anti-GFP antibodies; Table S3), iDISCO was performed with the no-methanol version of the protocol, which also gives good results (see Figure 4C for anti-PECAM staining) ...
In the HTS adaptation of the AO staining assay, the time elapsing between gametocyte activation and automated image acquisition is of the order of 1–2 h. This makes the AO-GCT assay particularly suited to monitor the viability of female gametocytes, as the activated male gametocytes ...
(Becton, Dickinson and Company, Franklin Lakes, NJ, USA). These samples were routinely monitored for at least 144 h. The bottles that tested positive were removed and subjected to Gram staining. The specimens were then inoculated into 5% sheep blood agar and BTB agar media (Nissui ...