(PI)-staining protocol and flow cytometry[17]. The distributions of 40,000 nuclei were quantified using a FAC STARPlus, flow cytometer (Becton Dickinson, San Jose, CA) and ModFitLT Cell Cycle Analysis program (Version 2.0; Verity Software House, Topsham, ME). Statistical significance of ...
vary by cell type, cell cycle, and cell viability. Therefore, visualization of mitochondra is important. Since mitochondria have electron transport systems, they can be stained with various redox dyes. MitoRed and Rh123 readily pass through ...
Cell Staining Protocol 细胞染色介绍.pdf,Cell Staining Introduction Visualization of a cell with fluorescent compounds provides a important role in oxidative metabolism by generating ATP as wide variety of information for the analysis of cell functions.
Cell cycle analysis of A498 cell lines with PI staining using Flow cytometry.Akanksha, SrivastavaRatnakar, TiwariVikas, SrivastavaTej, Bali SinghRavi, Kumar Asthana
细胞周期检测试剂盒(Cell Cycle and Apoptosis Kit) 是一种采用经典的碘化丙啶染色(Propidium staining,即 PI staining)方法进行细胞周期与细胞凋亡分析的检测试 剂盒。 碘化丙啶(Propidium,简称 PI)是一种双链 DNA的 荧光染料。碘化丙啶和双链 DNA 结合后可以产生荧光, ...
Cell cycle analysis was performed using propidium iodide (PI; Roche, #11348639001) staining as described previously37,38. Cells were seeded at 50% confluence into 10-cm plates and treated with a vehicle or cell cycle inhibitors for 48 h. Next, cells were collected and fixed in cold 70% ...
别名:细胞周期与凋亡检测试剂盒(PI法) 细胞周期与凋亡检测试剂盒(PI法)(Cell Cycle and Apoptosis Analysis Kit (PI)产品及特点: 本产品是一种采用经典的碘化丙啶染色(Propidium Staining,即PI staining)方法进行细胞周期与细胞凋亡分析的检测试剂盒。其染色原理为:碘化丙啶是一种双链DNA的荧光染料,其和双链DNA...
Cell Cycle Assay Kit-PI/RNase Staining试剂盒,可快速完成细胞周期的分析,操作简便。本产品使用碘化丙啶 (PI) 作为DNA荧光染料,通过流式细胞术得到细胞周期中G0/G1,G2和S期细胞的比例。 所需的设备和材料 – 流式细胞仪(碘化丙啶PI:λex=535 nm,λem=617 nm) –37℃培养箱 –PBS缓冲液 –1.5 ml微量...
Regulation of the cell cycle. Immunostaining for phospho-histone H3 (PHH3) and incorporation of bromodeoxyuridine (BrdU) marks the mitosis (M) and synthesis (S) phases of the cell cycle. Ki67 staining is absent in quiescent cells. Association of E2F with Rb is disrupted when Rb is ...
细胞周期检测试剂盒升级版(Cell Cycle Assay Kit Plus)采用 RedNucleus I 染色法检测细胞周期,RedNucleus I 是一 种远红外核酸染料,具有细胞膜透性,可快速进入活细胞,特异结合 DNA,对活细胞进行周期检测,且无需 RNase 的消化。与传统的碘化丙啶染色(PI staining)方法相比,细胞无需破膜或固定,操作更加简便。