This chapter discusses the fact that chromatin modifications distinguish genomic features and physical organization of the nucleus. The spatial organization of genomic DNA in the nucleus thus represents the next frontier in functional genomics and epigenomics. The specific DNA sequences associated with a ...
which are part of both the nuclear membrane and the nuclear matrix, which creates a potential difference between them. The nuclear membrane is a four-layer phospholipid dielectric, so the potential vector
Cohen, D., Chumakov, I. & Weissenbach, J. A first-generation physical map of the human genome.Nature366
ATP dependent chromatin remodeling factors use the energy of ATP hydrolysis to introduce superhelical torsion into DNA and to alter the rotational phasing of the DNA on the surface of the histone octamer, thus to increase the accessibility of the nucleosomal DNA to transcription factors and ...
Fine structural observations on the development of the sperm head in the mouse The stages of spermiogenesis in the mouse were investigated with the electron microscope with emphasis on morphogenesis of the sperm head. The nucleus undergoes chromosomal condensation in the concluding stages of spermiogenesi...
The human genome is by far the largest genome to be sequenced, and its size and complexity present many challenges for sequence assembly. The International Human Genome Sequencing Consortium constructed a map of the whole genome to enable the selection o
Physical Biology is a peer-reviewed scientific journal published by IOP Publishing covering a range of fields that bridge the biological and physical sciences, including biophysics, systems biology, population dynamics, etc. The editor-in-chief is Herbert Levine of Rice University. The journal is ind...
Cyclic GMP-AMP synthase (cGAS) is a major cytosolic DNA sensor that plays a significant role in innate immunity. Upon binding to double stranded DNA (dsDNA), cGAS utilizes GTP and ATP to synthesize the second messenger cyclic GMP-AMP (cGAMP). The cGAMP t
As transcription is a probabilistic event, these probabilities will be governed in part by the physical organization within the nucleus; i.e. influenced by the physical topology of the chromatin nanoarchitecture (the spatial organization and polymeric folding of DNA, histones, and other conjugated ...
Cellular outlines were estimated by a circular expansion from the outlines of the nucleus. In addition, a second and larger expansion from the nuclei was performed to measure the local area around each single cell (local cellular background). Standard CellProfiler-based intensity, shape and texture...