结果表明,SLC13A3 敲除抑制多种细胞类型(包括肿瘤细胞和人类 T 细胞)对 ITA 的摄取(图 1F)。此外,流式细胞仪分析显示,在SLC13A3敲除的A549细胞中,ITA-ICG分子的平均荧光强度(MFI)降低(图1G)。此外,上清液的荧光强度测定显示,SLC13A3敲除可提高细胞外空间中的ITA水平(图1H),表明SLC13A3介导了ITA的摄取。接下...
(F)用BBR(5和10mmol/L)处理H157细胞24h,流式细胞术检测质膜PD-L1。PD-L1平均荧光强度(MFI)的统计数据如图右图(nZ3)。所示数据为三个独立实验的平均值,均值标准误差(SEM)。**P<0.01,***P<0.001比较DMSO组。 3.3.BBR在体外恢复...
The quantification and comparison of PD-L1 mean fluorescence intensity (MFI) was based on measurements from 10 representative fields per slide from each of three normal and IPF patients. *, P < 0.05 compared with normal controls. Full size image Elevated PD-L1 levels in experimental models...
MerTK通过增强MDSC招募和降低细胞毒性T细胞功能。 利用多重免疫荧光(mfIHC)评估接受抗PD-1/PD-L1治疗的HCC患者。在耐药组观察到较高的MerTK和SLC7A1表达,与MDSCs、gmdsc和mmdsc浸润增加相关。MerTK表达与SLC7A11表达和MDSC浸润呈正相关,提示MerTK可能有助于免疫抑制TME,从而降低抗PD-1/PD-L1治疗的疗效。 5. 抑...
(b) Histogram comparisons of PD-L1 MFI changes in splenic monocytes and macrophages following conditional deletion of STAT1 (2-way ANOVA with Tukey’s multiple comparisons test, * = p = 0.0475, *** = p < 0.0001, 2 experimental replicates). All data presented are means + /-...
100μl/孔加入用pbs稀释100倍的羊f(ab’)2抗人igg-fc(pe)(购自abcam),4℃孵育30min,pbs清洗两次。100μl/孔加入pbs重悬细胞,在cytoflex(bechman)流式细胞仪上进行检测并计算对应的mfi。 [0238] 在如上方法的测定实验中,实验结果如图1所示,本发明所有的纯化样品和cho-hpd-l1细胞均有结合活性,且部分纯化...
当含有人PD-1和免疫球蛋白G(IgG)Fc片段的融合蛋白(25)与表达PD-L1的RKO细胞一起培养重量流式细胞术显示突变体的平均荧光强度(MFI)显著降低。这表明CRAC突变体PD-L1膜表达的降低导致PD-1结合能力的显著降低(图5E).总的来说,这些结果表明CRAC基序对于PD-L1和胆固醇之间的相互作用以及PD-1与PD-L1的结合至关...
19 细胞的染色显示出明显较高的平均荧光强度(MFI),这证明该抗体可结合至ro-Ll。明显的 FMI值(甚至在抗体的0. 01 μ g/ml的最低稀释度下)证明huro-Ll对结合ro-Ll蛋白的高 亲和力(表17A、17B和图2,右上)。当Huro-Ll抗体当在300. 19细胞中表达时展示出一 些结合人ro-L2或CLEC2D的能力(如由表18A、...
The PD-L1-positive cell percentage and the mean fluorescence intensity (MFI) of PD-L1 on H460 cells were greater than those for Calu-1 cells (Fig. 4A–C, p < 0.01). The western blot results revealed that PD-L1 protein expression on H460 cells was threefold higher than that on ...
Up, PD-L1 MFI: NSCLC-B, 2210; NSCLC-B CL1, 361; NSCLC-H, 501; bottom, PD-L1 MFI: NSCLC-B, 1600; NSCLC-B CL2, 990; NSCLC-H, 657. B Comparison of cell growth curves. Bars represent mean with SEM. Cell population doublings during 168 h of culture in 2D-growth adherent ...