Who We Are Cicadea Biotech provides custom PCR testing strategies and services for microbe detection and gene detection. We also serve as a Contract Research Organization for corporate and government clients. The lab is located at the Helix Center, a Biotech Incubator in St. Louis, Missouri, USA...
The emergence of SARS〤oVand the clinical syndrome of COVID19 resulted in a major decrease in transplant volumes during the first months of the pandemic followed by a change in practice, with strict screening and SARS〤oVPCR testing prior to transplant. Protocols required a negative SARS〤oVPCR...
Additionally, the first thirty-four of these specimens were used for testing in various RT-PCR instruments with all 3 assays to establish a proof of concept on the applicability of the assay in other RT-PCR instruments. The RT-PCR instruments used were a, a Bio-Rad CFX96 (Bio-Rad, ...
Accordingly, a provisional clinical diagnosis based solely on the respiratory signs must be confirmed by laboratory testing. Furthermore, rapid and accurate identification of these viruses is critical for the control of the diseases they cause. Therefore, the development of rapid, highly sensitive and...
Figure S5 reports post-test probability after successive PCR and BDG testing assuming complete conditional independence of both tests. Intensivists’ priors Overall, 25 physicians were interviewed before and after presentation of our results. Of them 5 had published a manuscript on Pneumocystis pneumonia...
Herein, we further expanded the previous work to include a total of 5 autoantibodies in the ADAP assay (IAA, GADA, IA-2A, ZnT8A and TGA) and conducted the 5-plex testing on a modified version of the Hamilton MicroLab STAR to achieve full automation. Briefly, 4 μL of serum samples ...
The detection limit of the real-time PCR assay of 20 TCID50/ml is comparable to the sensitivity of cell culture-based virus isolation, and was confirmed by the testing of EVEX positive and negative field isolates. It is important to remember, however, that PCR-based techniques only demonstrat...
How is molecular, PCR-based testing complementary to other kinds of testing? Cross-reactivity reduces the specificity of many serological tests: a positive result can be caused by organisms other than the target. Molecular assays, on the other hand, are highly specific because they detect genetic...
Marshall GM, Haber M, Kwan E, Zhu L, Ferrara D, Xue Cet al. Importance of minimal residual disease testing during the second year of therapy for children with acute lymphoblastic leukemia.J Clin Oncol2003;21: 704–709. ArticleGoogle Scholar ...
A tandem PCR method provided herein accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE-stabilized saliva or other DNA containing samples as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. As a model for such “raw...