However, several reports have suggested that this test has a high false-negative rate in patients with low virus levels.15, 16, 17 To address the issues with time to results of assays for SARS-CoV-2, previously developed methodologies for extremely rapid RT,18 as well as PCR, were ...
供货周期 现货货号 OneStep Ahead RT-PCR Kit(货号:220211) 50te bio-rad代理上海易汇生物科技有限公司OneStep Ahead RT-PCR Kit(货号:220211) 50test/盒详情介绍 上海易汇生物科技有限公司 biorad 12001925 biorad上海代理 biorad 1864008biorad 1863009biorad 12001925biorad 1863024 ...
The test includes an internal control (IC) to avoid false negative results. Study design: The system was established and validated using wild-type (WT) non-infectious synthetic RNA representing a fragment of the 3′ non-coding region of the TBEV genome. In addition, synthetic RNA differing ...
Temperature of the RT reaction. SUPERSCRIPT II RT has improved temperature stability compared to M-MLV-RT (Gerard, G., et al.,FOCUS14:91 (1992)). To test the effect of temperature on RT-PCR products, 36 primer sets (representing different genes from human, rat, plant, and one in vitro...
A total of 31 lyssaviruses (19 identified as classical RABV, 7 as EBLV-1, 4 as EBLV-2, and 1 as DUVV) were used to test the diagnostic performance of the PCR (Table1). All viruses used were detected with the expected band size in the hnRT-PCR technique (Table4). The specifici...
Validation of reference genes has been simplified with the design of statistical algorithms, such as geNorm and NormFinder, which not only test the expression stability of reference genes, but can also determine the number of reference genes required to provide accurate normalisation [17, 22]. This...
The real-time RT-PCR assay was further evaluated by testing field samples of European eels from the Netherlands, which were positive or negative for EVEX by virus isolation followed by an indirect fluorescent antibody test. The real-time RT-PCR assay allows rapid, sensitive and specific ...
To compare results between different groups of patients, the Mann–Whitney U-test was used for non-parametric variables and student’s t-test for parametric. 3. Results 3.1. Specificity of the RSV RT-PCR assay The specificity of the RSV RT-PCR assay was assessed by testing RNA or DNA ...
Statistically significant differences (Student’s t test for equal variance sample sets) are shown along with corresponding p values. Error bars represent standard deviation. (A) low MNV concentration. (B) high MNV concentration. 3.2. Removal of Potential RT-PCR Inhibitors In order to improve ...