(Invitrogen Corp., Carlsbad, CA, USA), 0.5μM Primer Mix containing 0.5μmol/l each of forward primer 5′-ATGAAGGCCTTCGAGTCCCTCAAGTCC-3′, with a 5′ 6FAM fluorescent tag, and reverse primer 5′-CGGTGGCGGCTGTTGCTGCTGCTGCTGCTG-3′ (Eurofins Genomics LLC, Louisville, KY, USA), and...
The primers were synthesized at Eurofins genomics (Louisville, KY, USA). Download: Download high-res image (293KB) Download: Download full-size image Fig. 1. Development of the 3′:5′ assay. (a) Schematic representation of rat Pgk1 mRNA showing the locations of the 5′ and 3′ ...
As a first test of these random primers we employed DNA plasmids of known sequence. We found that this mixture of primers enabled a continuum of discrete PCR products to be amplified from 4 different circular DNA plasmids (Figure2A). We shot-gun cloned these PCR products and confirmed that ...
Open AccessArticle by Sarah-Jo Paquette 1,†, Dominic Czekay 1,†, Jessica Manalaysay 1,2, Tara Furukawa-Stoffer 1, Aruna Ambagala 3, Stacey Vigil 4and Nariman Shahhosseini 1,5,* 1 Centre for Vector-Borne Diseases, National Centre for Animal Diseases, Canadian Food Inspection Agency,...