pBR322载体序列 ORIGIN 1TTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGGTAGTTTATCACAGTTAAA 61TTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGG 121CACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTT 181GCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATA 241TGCGTTGATGCAATTTCT...
(Bolivar, 1988). Although other cloning vectors are used more now, pBR322 was a very important early vector. pBR322 was made by combining segments of DNA from other plasmids. It has the origin of plasmid replication of a clinically isolated plasmid, pMB1, which is closely related to Col ...
pBR322 origin是质粒pBR322的复制区序列，在大肠杆菌中，质粒的复制起始，是从这个位点开始的。lacI是乳...
This chapter discusses pBR322, which is is a 4,362-bp double-stranded DNA plasmid cloning vehicle designed to allow simple and rapid preparation of cloned recombinant DNA fragments. It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites ...
A non-conjugative plasmid (constructed by Bolivar & Rodriguez) of 4.3 kb, can be mobilized by helper plasmids because (although it lost its mobility gene) it retained the origin of conjugal transfer (see Fig. P33 ). It is one of the most versatile cloning vectors with completely known ...
ColE1-derived plasmid replication origin 2534-3122 pBR322 DNA (4361bp)的图谱如下: pBR322 DNA中没有的酶切位点(Restriction enzymes that do not cut pBR322 DNA)包括: Acc65I AflII AgeI AleI ApaI AscI AsiSI AvrII BaeI BbvCI BclI BglII BlpI BmgBI BsaXI BseRI BsiWI BsrGI BssHII BstBI Bs...
Deletion of the cruciform binding domain in CBP/14-3-3 displays reduced origin binding and initiation of DNA replication in budding yeast[J]. BMC Mol Biol, 2007, 8 :27. [16]Zannis-Hadjopoulos M, Yahyaoui W, Callejo M, et al. 14-3- 3 cruciform-binding proteins as regulators of ...
This chapter discusses pBR322, which is is a 4,362-bp double-stranded DNA plasmid cloning vehicle designed to allow simple and rapid preparation of cloned recombinant DNA fragments. It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites for...
ColE1-derived plasmid replication origin 2534-3122 pBR322 DNA (4361bp)的图谱如下: pBR322 DNA中没有的酶切位点(Restriction enzymes that do not cut pBR322 DNA)包括: Acc65I AflII AgeI AleI ApaI AscI AsiSI AvrII BaeI BbvCI BclI BglII BlpI BmgBI BsaXI BseRI BsiWI BsrGI BssHII BstBI Bs...
ColE1-derived plasmid replication origin 2534-3122 pBR322 DNA (4361bp)的图谱如下: pBR322 DNA中没有的酶切位点(Restriction enzymes that do not cut pBR322 DNA)包括: Acc65I AflII AgeI AleI ApaI AscI AsiSI AvrII BaeI BbvCI BclI BglII BlpI BmgBI BsaXI BseRI BsiWI BsrGI BssHII BstBI Bs...