This chapter discusses pBR322, which is is a 4,362-bp double-stranded DNA plasmid cloning vehicle designed to allow simple and rapid preparation of cloned recombinant DNA fragments. It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites ...
To answer the question regarding the functions of specific components in the cloning vector pBR322, we will discuss each component one by one:1. Function of Ori (Origin of Replication): - Definition: Ori sta
This chapter discusses pBR322, which is is a 4,362-bp double-stranded DNA plasmid cloning vehicle designed to allow simple and rapid preparation of cloned recombinant DNA fragments. It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites for...
Plasmids are not a part of the genome and the same plasmid can exist in different species and gets transferred from one another Plasmids have their own origin of replication (ORI) and they replicate along with the cell so that each daughter cell possesses a copy of the plasmid also ...
pBR322 is an E. coli plasmid cloning vector containing the origin of replication from pMB1 (a plasmid in the ColE1 compatibility group; 1–3). The rop gene product, which regulates plasmid replication by stabilizing the interaction between RNAI and RNAII transcripts, maintains the copy ...
In the presence of RNA polymerase and DNA gyrase, specifically initiated RNA II (the leading-strand primer precursor) can form an RNA-DNA hybrid with the template that starts just upstream of the origin of DNA replication and continues for about 3 kilobases. Subsequent digestion of the RNA in...
elimination of small, multicopy, relaxed plasmids (pBR322 and pBR329 with CoLE 1 origin of replication in Escherichia coli , whereas plasmids of IncF 1 , H 1 and X group were totally refractory under similar conditions, suggesting that this agent was specific in curing the CoLE 1 group of ...
The potential of the cruciform structure formation as an important factor influencing p53 sequence-specific binding to natural DNA targets[J]. Biochem Biophys Res Commun, 2010, 391(3):1409-1414. [31]Zannis-Hadjopoulos M, Sibani S, Price GB. Eucaryotic replication origin binding proteins[J]. ...
One hypothesis on the cause of the exclusion effect is based off the size of the two plasmids. Because pBr322 and pUC19 both contain the same origin of replication, originally from pMBI (1), the bacterium does not distinguish between the two for purposes of replication. In addition, as ...
pBR322 and pUC19 are two types of plasmid vectors. They are popular cloning vectors forcoli. Both plasmid vectors have the same origin of replication form of plasmid pMB1. These vectors have multiple restriction sites. Both plasmid vectors have an ampicillin resistance gene as a selectable marker...