(Bolivar, 1988). Although other cloning vectors are used more now, pBR322 was a very important early vector. pBR322 was made by combining segments of DNA from other plasmids. It has the origin of plasmid replication of a clinically isolated plasmid, pMB1, which is closely related to Col ...
vectors. pbr322 it was the first artificial cloning vector created in the laboratory by francisco bolivar and raymond l.rodriguez. also, pbr322 is bigger in size compared to its puc counterparts. thus, they have a slower rate of replication. this e.coli cloning vector has the origin of ...