To further explore the functional relationship between PARP1 and CHK2, this study employed HOto induce an oxidative DNA damage response in cells. Here, we showed that CHK2 and PARP1 interact in vitro and in vivo through the CHK2 SCD domain and the PARP1 BRCT domain. Furthermore, CHK2 ...
[3] R.X. Huang, P.K. Zhou, DNA damage response signaling pathways and targets for radiotherapy sensitization in cancer,Signal Transduct Target Ther, 5 (2020) 60. [4] J. Murai, Y. Pommier, BRCAness, Homologous Recombinat...
Peng, B., R. Shi, J.Bian, Y. Li, P. Wang, H. Wang, et al., PARP1 and CHK1 coordinate PLK1 enzymaticactivity during the DNA damage response to promote homologousrecombination-mediated repair.Nucleic Acids Res(2021) 49...
IMPDH2 scored as a common interactor between PARP1 and TOP2A (Fig.4Aand Supplementary Data5). This evidence strengthened our hypothesis of a possible role of chromatin-associated IMPDH2 in the DNA damage response, given that PARP1 and TOP2A are chromatin master regulators of the DNA ...
inducedDNAdamageresponseinhumanbronchialepithelia1fHBE)cellsandtoinvestigatethemechanismof formaldehydecarcinogenicity.MethodsTheproteinlevelsweremeasuredbyWesternblot.Theinteraction betweendifferentproteinswasdeterminedbyco—immunoprecipitationassay.Thechemica¨nhibitorwasusedto ...
is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Poly-ADP-ribosylation (termed PARylation) is the addition of linear or branched chains of ADP-ribose. PARP1 participates in DNA repair by non-homologous end joining (NHEJ), homolo...
The prime function of nucleoli is ribogenesis, however, several other, non-canonical functions have recently been identified, including a role in genotoxic stress response. Upon DNA damage, numerous proteins shuttle dynamically between the nucleolus and the nucleoplasm, yet the underlying molecular ...
DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA...
Description PARP1, poly (ADP-ribose) polymerase 1, is an enzyme that catalyzes poly (ADP-ribosyl)ation of proteins, which initiates cellular response to DNA damage (PMID: 15743666). PARP1 dysregulation has been identified in acute myeloid leukemia, breast and colorectal cancers (PMID: 25483710,...
More than just a focus: The chromatin response to DNA damage and its role in genome integrity maintenance. Nat Cell Biol. 2011;13(10):1161–9. Article PubMed CAS Google Scholar Mah LJ, El-Osta A, Karagiannis TC. gammaH2AX: a sensitive molecular marker of DNA damage and repair. ...