Comparison of the set of targets determined by the experimental assay (PAR-CLIP) and computational methods (ElMMo, TargetScan 5.1) We computed the number of seed matches to each of the top 5 expressed miRNA families in the top 1000 CCRs from the AGO-PAR-CLIP. For each of these 5 miRNA ...
RNA-EMSA(RNA electrophoretk:mobility shiftassay,RNA凝胶迁移实验)是一种在体外研究RNA与蛋白质相互作用的常用技术。这项技术是基于RNA-蛋白质复合体在聚丙烯酰胺凝胶电泳(PAGE)中有不同迁移率的原理。首先,经过标记的RNA探针与纯化的蛋白质一起孵育,使其发生相互作用,并且结合。然后通过非变性聚丙烯酰胺凝胶电泳分离...
Post-transcriptional gene regulation is robustly regulated by RNA-binding proteins (RBPs). Here we describe the collection of RNAs regulated by AUF1 (AU-binding factor 1), an RBP linked to cancer, inflammation and aging. Photoactivatable ribonucleoside-e