Here we describe protocols for RNA Immunoprecipitation-Sequencing (RIP-Seq) and Photoactivatable-Ribonucleoside-Enhanced Cross-linking and Immunoprecipitation combined with deep sequencing (PAR-CLIP-Seq) to identify RNA targets of RNA-binding proteins (RBPs) on a transcriptome-wide level, discussing ...
PAR-CLIP(photoactivatable-Tibonucleoside-enhanced crosslinking and immunoprecipitation,光活化增强的交联免疫沉淀)是一种新的高通量检测与特定蛋白相互作用的RNA的位点实验技术。PAR-CLIP利用一类具有光活性的核糖核苷类似物(如4-硫尿核苷,4-SU)在细胞培养过程中掺入到新转录的RNA中,活细胞在紫外光(365nm)照射时,RNA...
Here we present an updated and condensed step-by-step PAR-CLIP protocol followed by the description of our RNA-seq data analysis pipeline. 展开 关键词: Next-generation sequencing PAR-CLIP Suite RNA recognition element Ribonucleoprotein DOI: 10.1007/978-1-4939-7540-2_5 ...
RNA-binding proteins (RBPs) establish posttranscriptional gene regulation (PTGR) by coordinating the maturation, editing, transport, stability, and translation of cellular RNAs. A variety of experimental approaches have been developed to characterize the RNAs associated with RBPs in vitro as well as ...
2. Mapping the Transc riptome-Wide Landscape of RBP Binding Sites Using gPAR-CLIP-seq: Experimental Procedures.Methods Mol Biol. 2016;1361:77-90.
2.Mapping the Transc riptome-Wide Landscape of RBP Binding Sites Using gPAR-CLIP-seq: Experimental Procedures.Methods Mol Biol. 2016;1361:77-90.
:exclamation: This is a read-only mirror of the CRAN R package repository. wdpar — Interface to the World Database on Protected Areas. Homepage: https://prioritizr.github.io/wdpar/, h
(axis.title = element_blank(), legend.position = "bottom") ``` - + If you need to calculate protected area coverage statistics for a country, please note that you will need to manually clip the cleaned @@ -238,17 +240,11 @@ vignette](https://prioritizr.github.io/wdpar/articles/...
xpd: 剪切图片, 可以参看 clip 函数. 如果 xpd 值为 FALSE, 所有的图形被剪切为绘图区域 (plot); 如果 xpd 值为 TRUE, 所有图形被剪切为图像区域 (figure) (绘图区域和边缘 (margin)); 如果 xpd 值为NA, 则图形被剪切为设备区域, 即图像区域和外边界(outer) (参看下面图像区域图片). ...
概述 别名 Thrombin 宿主 Rabbit 反应种属 Human 应用 WB: 1:1000 免疫原 Recombinant protein 性能 形式 Liquid 浓度 0.5 mg/mL 纯化方法 Protein A affinity column 类型 Monoclonal Antibody 克隆号 293-58 储存/保存方法 Store at -20℃ for one year. ...