2.Mapping the Transc riptome-Wide Landscape of RBP Binding Sites Using gPAR-CLIP-seq: Experimental Procedures.Methods Mol Biol. 2016;1361:77-90.
2. Mapping the Transc riptome-Wide Landscape of RBP Binding Sites Using gPAR-CLIP-seq: Experimental Procedures.Methods Mol Biol. 2016;1361:77-90.
由于RNA-蛋白质复合物结构利用晶体衍射法或NMR解析通常非常困难,并且成本高,而该方法不需要超高的蛋白纯度和复杂的结晶过程,数据分析也相对简单。 5.PAR-CLIP PAR-CLIP(photoactivatable-Tibonucleoside-enhanced crosslinking and immunoprecipitation,光活化增强的交联免疫沉淀)是一种新的高通量检测与特定蛋白相互作用的RNA...