Genetic strategies for the generation of PA-GFP::NLS expressing mice.Manuel PeterBrice BathellierBruno FontinhaPinelopi PliotaWulf HaubensakSimon Rumpel
The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located ...
Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. 仅用于科研。不用于诊断过程。未经明确授权不得转售。
Ordering Please select your country: Size Detection pAAV-GFP Control Vector Catalog Number AAV-400 Size 10 µg Detection N/A Manual/Data SheetDownload SDSDownload MapDownload SequenceDownload Product Details Use this control vector toco-transfectalong withAAVpackaging vectorsto produce a recombinantAAV...
pAAV-CAG-ArchT-GFP catalog : 29777 more info or order: Addgene product webpage citations: 17 Reference La Terra D, Bjerre A, Rosier M, Masuda R, Ryan T, Palmer L. The role of higher-order thalamus during learning and correct performance in goal-directed behavior. elife. 2022;11:pub...
Wild-type and psme-3,GFP(OE) worms were raised at 20 °C during development and then grown at the indicated temperatures until day 6 of adulthood. Statistical comparisons were made by two-tailed Student’s t-test for paired samples. P value: *P < 0.05, **P < 0.01, ***P...
pGEM-Prm1-EGFP was liberalised using AseI and PvuII. The resulting 2.7 kb fragment was purified and microinjected into oocytes of BDF1 mice. Transgenic founder were identified be performing PCR on genomic DNA isolated form tail biopsies using following primers (GFP290-5′—GCACGACTTCTTCAAGTC...
1a). Using western blotting, we detected the modification of GFP::LGG-134, and found a significant increase in the ratio of phosphatidylethanolamine (PE) conjugated GFP::LGG-1 (PE-GFP::LGG-1) to nonlipidated GFP::LGG-1 in worms at low temperature (Fig. 1b). The increase in LGG-1 ...
a, Filter trap analysis of day 6 adult worms that express polyQ67::YFP or control polyQ19::CFP (detected by anti-GFP antibody) in neurons. Representative of three independent experiments.b, Western blot of day 6 adult worms to assess total polyQ67::YFP and polyQ19::CFP levels (detected ...
VHH Fc fusion 111In labeling APPswe/PS1dE9 mice Biodistribution SPECT 1. Introduction Due to their long, protruding and highly variable CDR3 section, llama derived single domain antibody fragments (VHH) have the ability to access epitopes normally hidden for conventional antibodies. As such they ...