The aims of this study were to determine 1) whether sex affectsRBC survival; 2) whether RBC survival differs between the biotin method and an alternative method that uses GFP; and 3) whether repeat exposure of mice to biotin results in an antibiotin antibody response or decreased RBC survival...
The aims of this study were to determine 1) whether sex affects RBC survival; 2) whether RBC survival differs between the biotin method and an alternative method that uses GFP; and 3) whether repeat exposure of mice to biotin results in an antibiotin antibody response or decreased RBC ...
Although GFP fluorescence of Ubb+/− MEFs also increased following tBHQ treatment (Fig. 2A), Ubb was only slightly upregulated in both control and Nrf2−/− MEFs (Fig. 2B, left). Therefore, under As(III) exposure, upregulation of Ubc, but not Ubb, is mediated by activation of the...
6c. d P6/7 UBC13 loxP/loxP mice were electroporated with the Cre expression plasmid or control vector together with the GFP expression plasmid and analyzed as in Fig. 1a. Cre-induced knockout of UBC13 in granule neurons increased presynaptic bouton density in vivo (***p < 0.001, t...
When IR1+2 tagged with green fluorescence protein at its amino terminus (GFP-IR1+2) was transfected into COS-7 cells, we found that ∼90% of the nuclear Ubc9 was sequestered in the cytoplasm. We also observed that both SUMO-1 and SUMO-2/3 were mislocalized, and promyelocytic ...
(Fig.6a, d). To address whether Ubc9 indeed regulates the M2 program via SUMOylation of IRF4, BMDMs were transduced with Ad-IRF4-WT or Ad-IRF4-K349R and then subjected to IL-4 induction for 24 h, followed by flow cytometry analysis by gating on GFP+cells. Of note, the ...
Moreover, the Ubc9 adenovirus group displayed increased numbers of yellow plus red puncta and a rising ratio of red to yellow puncta on the mRFP-GFP-LC3 fluorescence assay, indicating that Ubc9 induces an acceleration of autophagic flux from activation to degradation. Mechanistically, Ubc9 up...
Strikingly, STAT3 deficiency in humans and mice also associates with bone abnormalities and elevated osteoclast amounts5,8,9 suggesting effects on the receptor activator of nuclear factor kB (NF-kB; RANK) signalling pathway. By contrast, chronic STAT3 activation in tumour micro- environments induces...
(h) HR repair assays of U2OS_DR-GFP cells transfected with miR-205 alone or in combination with ZEB1 or Ubc13, or both. n ¼ 3 wells per group. Data in b,d,f,g,h are the mean of biological replicates from a representative experiment, and error bars indicate s.e.m. Statistical ...
HeLa cells were transfected with an expression vector encoding GFP-SUMO-1 followed by the isolation of ER enriched microsomes. Anti-GFP antibodies were used to pull down GFP-SUMO-1 followed by Western blot analysis with anti-GFP antibodies (to report the presence of GFP-SUMO-1) and anti-...