CO 2 -sensitivity of GABAergic neurons in the ventral medullary surface of GAD67-GFP knock-in neonatal mice . Adv Exp Med Biol 605 : 338–342, 2008. [ PubMed ]Kuribayashi J, Sakuraba S, Hosokawa Y, Hatori E, Tsujita M, Takeda J, Yanagawa Y, Obata K, Kuwana S. CO2-sensitivity of...
However, it is unknown whether these mice have a normal sleep phenotype. Compared with wild-type control mice, GAD67-GFP knock-in mice had the same amount of non-rapid eye movement (NREM) sleep and rapid-eye movement (REM) sleep, a similar diurnal distribution of sleep, no NREM or REM...
GFP转基因小鼠 1. Methods Bone marrow cells isolated fromGFP transgenic miceare directly planted in culture flask and an exchange of the total volume medium is made at different time. 方法分离GFP转基因小鼠骨髓细胞直接种植在培养瓶中,分别于2h、4h、6h、8h、10h、12h、14h、16h、24h和48h进行首次...
γ氨基丁酸(GABA)是脊髓背角,前角内主要的抑制性神经递质.为了更好地观察脊髓背角内GABA能神 经元的形态和功能,本研究使用了两种谷氨酸脱羧酶67绿色荧光蛋白(GAD67-GFP)基因敲入小鼠,并观察了敲入小鼠脊髓内的GFP表达状况.用免疫 荧光组织化学双标记方法显示脊髓内所有的GFP阳性神经元基本上都呈GAD67和GABA阳性;GFP...
Maximal number of the cleaved caspase-3 stained interneurons (16.6 % of the total number of GFP expressing neurons and 21.6 % of the total number of caspase-3 stained cells) was observed in the hippocampal slices from P6-9 mice, and minimal damage to interneurons was observed in P3–4 and...
Because the GAD67 protein was absent in the homozygous GAD67-GFP (Δneo) mouse brain (data not shown), we used GAD67-GFP (Δneo) mice as GAD67 KO mice. The GADs DKO mice were obtained by crossing GAD65 KO and GAD67 KO mice. Wild-type mice derived from littermates were collected...
Here, we investigate the functional role of GAD67 in an AD mouse model withGad67haploinsufficiency that is caused by replacing one allele ofGad67with green fluorescent protein (GFP) gene during generation of GAD67-GFP mice. To genetically reduce GAD67 in AD mouse brains, we crossed theGad67...
Quantification and characterization of GABA-ergic amacrine cells in the retina of GAD67-GFP knock-in mice. Acta Ophthalmologica. 2008; 86:395- 400. [PubMed: 17995983]May CA,Nakamura K,Fujiyama F,et al.Quantification and char-acterization of GABA-ergic amacrine cells in the retina of GAD67...
GFP expression covers the whole developmental stage of newborn neurons, beginning within the first week of cell division and disappearing as newborn neurons mature, about 4 weeks postmitotic. Thus, the GAD67-GFP transgenic mice provide a useful genetic tool for studying the development and ...
Mouse GAT4 immunoreactivity in circumvallate papillae from GAD67-GFP mice.Margaret R. StarostikMichelle R. RebelloKellie A. CotterAkos KulikKathryn F. Medler