2c). However, the ability of p62 to assemble condensates was impaired in Nur77−/−HeLa cells, which was restored by reexpressing GFP-Nur77. Thus, celastrol-induced formation of p62 condensates is dependent on Nur77 expression. As the liquidity of condensate is a critical determinant for ...
cells were treated with or without (untreated) 10 μM forskolin in DMEM supplemented with 1% (v/v) FBS for 24 hr. Phase contrast (Phase) and GFP-expression (GFP) images were detected
Results Results from knockout mice implicate Nur77 in persistent UPEC bladder infection Given the importance of Nur77 in multiple cellular processes that are known to be involved in the UPEC UTI pathogenic cascade, and the results from our prior rUTI model36, we reasoned that Nur77 might ...
To further elucidate the role of Nur77 in macrophage physiology, we compared the transcriptome of bone marrow-derived macrophages (BMM) from wild-type (WT) and Nur77-knockout (KO) mice. Results In line with previous observations, SDF-1α (CXCL12) was among the most upregulated genes in ...
Prolonged cardiac NR4A2 activation causes dilated cardiomyopathy in mice Sadia Ashraf Heinrich Taegtmeyer Romain Harmancey Basic Research in Cardiology(2022) Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia ...
R.N.H. and L.M.C. designed and did experiments, analyzed data and contributed to the writing of the manuscript; H.G.H. did experiments with NR4A1-GFP mice; D.N. and A.M.G. did experiments; J.A.P. conceived of the studies of NR4A1-GFP mice, analyzed data and contributed to ...
To test whether retinoids could also induce Nur77 in mouse thymocytes, thymocytes isolated from 4 week old mice were exposed to ATRA and 9cRA, and Nur77 expression was detected on both mRNA (Fig. 1A) and protein levels (Fig. 1B). As seen in Fig. 1, both ATRA and 9cRA were able ...
GFP-Nur77, which was found exclusively in the nuclei of control cells, displayed a speckled pattern in the cytoplasm of cells treated with celastrol and TNFα (Figure S3C). In contrast, the combination treatment had no effect on the nuclear localization of transfected Myc-RXRα (Figure S3D),...
F, Kit+ UAS-GFP bone marrow cells were transformed with MLL-AF9 retrovirus, transduced with Gal4-Nur77 retrovirus, and engrafted into recipient mice. G, summary ratios of GFP+mCherry+ cells relative to total mCherry+ cells in the bone marrow cells, peripheral blood, spleen, and peritoneal...
The population, of GFP positive cells that corresponds to the cells transfected with either the control vector pEGFPC1 or with GFP-HGV-APT were gated and analyzed among the whole population of cells, by staining cells with PO-PRO™-1 and 7-AAD apoptotic cells show violet/blue fluorescence,...