We investigated, in two p53 null cell lines, the human SCLC line H1299 and a subline derived from the human colon carcinoma cell line HCT116 (HCT116/379.2), the effects of Np73卤 overexpression on cell growth and the response to anticancer treatment. We generated three different clones ...
expression, the FRO cell line should obviously not be called a “p53-null”. Many examples of this type of situation can be found in the literature. Another reason why “p53-null” should be used cautiously to describe cell lines that express mutant p53 is the observation that p53 muta...
突变体p53能够通过内源性的PDGFRb促进胰腺癌的转移 Journal:CellReporter:XuWangIF:33.116 Abstract antiproliferativemetastasis Mutantp53 prometastaticphenotype p73/NF-Y vivoPDGFRb metastasis × vitroinvasion prognosticmarker Background Induceapoptosis Promotemetastasis p53 drugresistance mutantp53 preventangiogenesis ant...
Cell lines expressing wild-type, DNA-contact hotspot mutants, null (undetectable p53 mRNA or protein), splicing defects, truncated, or frame-shift p53 were classified as lines presumably insensitive to the desired compounds. The remaining TP53-mutated cell lines, for whom the potential of being ...
SKOV3 and MDA MB 436 are p53-null cell lines and express high levels of MELK. As shown in Fig. 3a–d, induction of WT p53 by Dox treatment significantly reduced MELK protein levels in both cell lines, while increasing p21 protein levels (a downstream marker of WT p53). In addition, ...
EMT induction by mutant p53-R273H was also confirmed in other cells with wild-type or null p53 (Supplementary Fig. S2). Furthermore, the introduction of mutant p53-R273H led to resistance to EGFR-TKIs in two cells (Fig. 4b). Lastly, we examined migratory and invasive potential, which ...
A p53-null anaplastic thyroid carcinoma cell line, FRO, and a FRO subline stably expressing a temperature-sensitive (ts) mutant of p53 (p53Val138), tsFRO, were used. ts-p53 functions as mutant and wt at nonpermissive (37 C) and permissive (32 C) temperatures, respectively. tsFRO showed...
Dermal papilla cell linesp53 knockoutWe have attempted to establish a cell line from hair follicle dermal papillae microdissected from vibrissa of mice lacking p53 tumor suppressor gene. The hair follicle dermal papillae were obtained from three types of mice; null knockout, hetero-knockout and ...
The p53 null HL-60 cell line was transfected with plasmids coding for either the wild-type p53 or mutant p53 gene. The stable expression of wild-type p53 resulted in a significant increase in sensitivity to the topoisomerase II poisons etoposide and doxorubicin, but not to the topoisomerase II...
p53 deficiency supports cell proliferation though Asn p53-null mice predominantly develop and succumb to lymphomas9,10,17. We thus hypothesised that there might be some advantage(s) provided by p53 deficiency that can facilitate tumourigenesis in plasma. To test this hypothesis, we transplanted lucife...