Because p53 and DREAM are known to up- and down-regulate most p53-dependently regulated genes, respectively, mouse p53 and E2f4 binding data from genome-wide chromatin immunoprecipitation (ChIP) experiments was integrated. Binding of the key DREAM complex component E2f4 [5] served as a proxy fo...
Fig. 2: MDM2 and BET inhibitors cooperate to eradicate AML in in vivo mouse models. aPrimary murine Trib2 AML blasts, treated in vitro for 72 h with vehicle, 200 nM CPI203, 2.5 μM nutlin-3, or the drug combination. Cell death assessed by flow cytometry using Annexin-V and PI...
43、 embryogenesis but dispensable for p53 and Mdm2 stabilityJ. Proceedings of the National Academy of Sciences, 2011, 108(29): 11995-12000.31. Toledo F, Krummel K A, Lee C J, et al. A mouse p53 mutant lacking the proline-rich domain rescues Mdm4 deficiency and provides insight into the...
Mouse p53, rabbit polyclonal Ab Leica Biosystems Cat# P53-CM5P, RRID: AB_2744683 p53 antibody, rabbit polyclonal Ab Abcam Cat# ab131442, RRID:AB_11155283 beta actin, clone 2D1D10, mouse monoclonal Ab Genscript Cat# A00702 KU80, rabbit polyclonal Ab Cell Signaling Cat# 2753, RRID: AB_...
In the co-culture of macrophage cells with mouse cancer cells experiments, the M0 macrophage cells (RAW264.7) were activated by different stimulatory cytokine cocktails to derive M1 macrophages and M2 macrophages. After being exposed to MC38 cell lysates that were treated with different stimulations...
Expression System was used, and the regulable cell line of the inducible expression of p53 gene was established; mRNA difference display technique was used to clone p53 downstream genes directly and in situ hybridization technique was used to initially understand their functions in mouse embryonic ...
(+) T cell infiltration and activation, while single copy loss of HIF1α or high levels of HIF2α mRNA expression correlate with altered immune microenvironments in human ccRCC [72]. However, researchers may want to keep in mind that mouse ccRCC tumor models may not always fully reflect ...
as a potent anti-cancer agent for HPV+ HNSCC. Fenofibrate induced the accumulation of the p53 tumor suppressor and re-programmed the tumor microenvironment to drive immune cell infiltration. We provide compelling evidence to reposition fenofibrate as a single agent or in combination with standard ther...
Anti-human p16, p53 were performed using a mouse monoclonal antibody [BP53-11] and Ki-67 [clone MM1 mouse monoclonal antibody]. Prepared slides were incubated for 32–40 min at room temperature after the inactivation of the endogenous peroxidases using primary antibodies in addition to the ...
the cells were incubated with primary antibody overnight at 4 °C. Then the cells were washed with PBS and incubated with flurochrome conjugated secondary antibody against mouse or rabbit (proteintech, Wuhan, Hubei, China) for 1.5 h at room temperature. The specimens were then stained nuclei wi...