TP53基因位点在肿瘤中的变异方面的研究进展,不但没有简化我们对p53变异如何促进肿瘤生成的理解,反而让我们的理解更为复杂化。TP53基因变异中最常见的也是被研究得最为彻底的是位于DNA结合蛋白域的错义突变(missense mutation)。这意味着p53的这一特征对肿瘤抑制非常关键。目前的教条是把p53分为野生型或者突变型两大...
Truncating mutation tumors were defined as tumors with TP53 frameshift, nonsense and splice site mutations. RNA-seq data and gene somatic mutations data from cancer cell lines was downloaded from Xena Browser (CCLE dataset, RPKM), filtered for large intestine cell lines and divided into groups ...
000 cancer patients is 42%. However, the mutation frequency varies across different types of tumors, with mutation frequency of 89.02% in small cell lung cancer
Mutant p53 has different structures compared with wild-type p53, which causes colon cancer cell progression and promotes tumor metastasis. Here, we showed that the PHLPP2-AKT pathway is a major target by which p53 promotes malignancy. In this research, we found p53 mutation could bind the ...
有时,黑化到极致的p53基因还会获得功能性突变(gain-of-function mutation),华丽丽地变身为一个“癌基因”,从多个角度给癌症的发生发展添buff,如增强肿瘤的侵袭性和耐药性等。虽然这些获得功能性突变可以有多种活性,但是最主要的规律是这些突变会对抗野生型p53的功能,或者加重p53缺失造成的结果。
They were used to treat H1299 cells (null for endogenous p53) transfected with p53-R175H, a prototypical structural p53 mutant allele as well as the most common p53 mutation (Figure 1A). Treated cells were assayed for reactivity to the well-characterized PAb1620 antibody (Figures 1F and S1...
目前,根据突变对DBD结构及蛋白整体功能的影响,mutant p53可分为两类:一类是会干扰p53与靶 DNA 结合的接触型突变(如:R273H、R248W、R282W和R248Q),即DNA接触面的氨基酸残…
Cell lines with p53 mutations were slightly more sensitive to cisplatin than those with wild-type p53. The type of mutation did not influence radio- or chemosensitivity. p14 ARF, an activator of p53, was lost or mutated in all cell lines. Three cell lines showed overexpression of HDM-2, a...
TP53 mutation 1.33 0.92–1.93 NS 1.84 0.90–3.77 NS Age at diagnosis 1.01 1.00–1.02 NS 1.02 0.98–1.05 NS Tumour size 1.02 1.01–1.03 0.001 1.04 1.02–1.06 0.001 ≥4 positive lymph nodes 1.49 1.06–2.09 0.02 1.57 0.81–3.02 NS ER (positive) 0.62 0.40–0.98 0.04 1.02 0.46–2.28 NS PR...
Cell 137, 413–431 (2009). Article CAS PubMed Google Scholar Petitjean, A. et al. Impact of mutant p53 functional properties on TP53 mutation patterns and tumor phenotype: lessons from recent developments in the IARC TP53 database. Hum. Mutat. 28, 622–629 (2007). Article CAS PubMed ...