1997年，顾伟教授首次发现p53的C端结构域 (C terminal Domain, CTD) 可以被乙酰化修饰从而促进其蛋白稳定。不仅如此，p53 乙酰化也可以通过阻止 MDM2 募集到 p53结合的靶基因启动子区来促进靶基因的表达。进一步研究发现，p53的DNA结合结构域 (DNA binding Domain, DBD) 的乙酰化修饰对于其转录活性至关重要：p53 ...
p53 DNA binding domain sequence alignment of Homo sapiens, Mus musculus, Xenopus laevis, Danio rerio, Drosophila melanogaster and Caenorhabditis elegans.Mattia LionIvan RaimondiStefano DonatiOlivier JoussonYari CiribilliAlberto Inga
1997年,顾伟教授首次发现p53的C端结构域(C terminal Domain, CTD)可以被乙酰化修饰从而促进其蛋白稳定。不仅如此,p53 乙酰化也可以通过阻止 MDM2 募集到 p53结合的靶基因启动子区来促进靶基因的表达。进一步研究发现,p53的DNA结合结构域(DNA binding D...
Defining the p53 DNA-binding domain/Bcl-x(L)-binding interface using NMR. FEBS Lett 2004 ; 559:171 - 174Petros, A., A. Gunasekera, N. Xu, E. Olejniczak, and S. Fesik. 2004. Defining the p53 DNA-binding domain/Bcl-XL-binding interface using NMR. FEBS Lett. 559 : 171-174....
p53的功能结构域包括:N端反式激活结构域,DNA结合结构域(p53-DBD, DNA binding domain),富含脯氨酸结构域,四聚体结构域,C端调控结构域。在超过50%以上的人类肿瘤中均发现了在p53-DBD上的突变,对p53-DBD的生化和结构分析表明p53是以动态且不稳定的...
DNA-binding domain (DBD) that is more thermostable than all vertebrate p53 DBDs tested so far, but not as stable as that of human p63. We also identified features associated with its increased thermostability. In addition, theA. pompejanahomolog shares DNA-binding properties with human p53 ...
p53基因位于17p13.1染色体,包含11个外显子及10个内含子区,编码的p53蛋白由N端转录激活结构域(Transactivation domain,TAD)、富含脯氨酸结构域(Proline-rich domain,PRD)、中央的DNA结合结构域(DNA-binding domain,DBD)、寡聚化结构域(Tetramerization domain,OD)和C末端调控域(C-Terminal domain,CTD)5大结构域组成...
近日,美国纽约哥伦比亚大学癌症遗传研究所顾伟课题组在《Science Advances》期刊上发表了题为“An unexpected role for Dicer as a reader of the unacetylated DNA-binding domain of p53 in transcriptional regulation”的研究论文。该研究揭示了一个全新的p53负调控蛋白—Dicer,其可以作为非乙酰化p53的识别蛋白,对于...
Mis-sense mutations affecting TP53 promote carcinogenesis both by inactivating tumor suppression, and by conferring pro-carcinogenic activities. We report here that p53 DNA-binding domain (DBD) and transactivation domain (TAD) mis-sense mutants unexpecte
We indicated that the prognostic significance of all types of mutations in the DNA-binding domain is not the same, and that the DNA-binding domain having a certain code structure is important for estimating the prognosis of breast cancer patients.Contact: keiko@is.noda.tus.ac.jp or hara@is....