In both cases, the p53 binding regions displayed a high degree of sequence homology with the consensus binding site defined for human genomic DNA. One of the clones was found to be derived from the LTR of a retrovirus-like element (a member of the GLN family). The region encompassing the...
Specific sequences in the human genome are the sites of strong binding of wild-type p53 protein, but not mutant forms of the protein. These sequences are used diagnostically to detect cells in which the amount of wild-type p53 is diminished. The sequences can also be used to screen for...
the 5’flanking region of theSCD1gene contains one site that matches the consensus p53-binding sequence (Fig.4k). ChIP analysis further demonstrated that endogenous p53 binds on the promoter region of theSCD1gene (Fig.4l). Moreover, Dox and nutlin-3, both can activate p53, could...
60 Binding and transactivation by the tumor suppression protein p53 are encoded in the differential structural properties of its response elements and Haran, T. E. 2011 . Sequence-dependent cooperative binding of p53 to DNA targets and its relationship to the structural properties of the DNA ......
Briefly, the DNA fragment containing the potential p53-binding region was amplified by PCR with primers used in ChIP assay and was cloned into a pGL3-promoter vector (Promega). In all, 293T cells were plated 18 h before transfection in 24-well plates and transiently transfected with 500...
Sequence specific DNA binding by p53 专利内容由知识产权出版社提供 专利名称:Sequence specific DNA binding by p53 发明人:Bert Vogelstein,Kenneth W. Kinzler,Michael I. Sherman 申请号:US08/299074 申请日:1994 0901 公开号:US05955263A 公开日:19990921 摘要:Specific sequences in the human genome are ...
The p53 transcription factor is either latent or activated through multi-site phosphorylation and acetylation of the negative regulatory region in its C-terminal domain (CTD). How CTD modifications activate p53 binding to target DNA sequences via its core domain is still unknown. It has been propo...
binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding P53-like...
A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein Genes Dev, 7 (1993), pp. 2565-2574 CrossrefView in ScopusGoogle Scholar Cho et al., 1994 Y. Cho, S. Gorina, P...
region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription cofactor binding protein C-terminus binding histone deacetylase regulator activity metal ion...