Methionine 358 in the plasma protein alpha 1-antitrypsin (alpha 1AT) is an oxidation-sensitive reactive-center residue critical for proteinase-inhibitory activity. Reaction of alpha 1AT with 20 microM to 1.67 mM cis-dichlorodiammineplati... SL Gonias,MW Swaim,MF Massey,... - 《Journal of ...
These reagents may be used in the presence of UV light, catalyst, ultrasound to advance the oxidation process. AOPs may be classified broadly into four groups: photocatalytic process (H2O2/UV, O3/H2O2/UV, UV/TiO2, H2O2/TiO2/UV, O3/TiO2/UV); the Fenton reaction-based processes (Fe2+/H2O2...
The fast reaction of peroxynitrite with CO2 and the high concentration of dissolved CO2 in vivo (ca. 1 mM) suggest that CO2 modulates most of the reactions of peroxynitrite in biological systems. The addition of peroxynitrite to CO2 prod... Stadtman,Earl R. - Springer US 被引量: 1747发表...
4188 Accesses 1 Citations 87 Altmetric Metrics details Abstract The majority of anticancer agents have a reduced or even complete loss of a therapeutic effect within hypoxic tumors. To overcome this limitation, research efforts have been devoted to the development of therapeutic agents with biological...
The final reaction mix was incubated for 5 min at 25 1C, followed by 1 h at 42 1C and another 15 min at 70 1C, and the volume was adjusted to 100 ml with ultrapure water. Two cultures grown on n-hexadecane and two cultures grown on sodium octanoate were used to prepare cDNAs ...
In LPO, three phases can be distinguished: the initiation, the propagation, and the termination phase.79,82LPO is initiated when a ROS encounters a PUFA (phase 1).83Together, they react to form a carbon-centered lipid radical. In the propagation phase, this newly formed lipid radical react...
This is confirmed by variation of the surface volume ratio. The surface reaction appears to be approximately first-order in propionaldehyde, and half-order in O 2, with a rate constant of 6.3 × 10 3 (litre mole 1) 1 2 sec 1. From the [CO 2] [CO] ratios and [CO], time profile...
Protein oxidation is defined here as the covalent modification of a protein induced either directly by reactive oxygen species or indirectly by reaction wi... SHACTER,EMILY - 《Drug Metabolism Reviews》 被引量: 732发表: 2000年 Reactive oxygen-mediated protein oxidation in aging and disease. Highly...
Lymphoblasts in the log-phase of growth were harvested by centrifugation for 10 min at 800 x g. The cells were washed twice with PBS and reisolated. After the final wash, cells were resuspended in a total volume of 0.3 ml of PBS supplemented with 0.1% glucose and 0.4 mg/ml defatted ...
The analysis was performed using triple detection conditions with either a GPC50 (Polymer Laboratories, UK; THF mobile phase) or a Malvern OmniSec System (Malvern, UK; DMF mobile phase). Molecular weight and molecular weight distribution of PPS polymers were determined using a PLgel 5 μm MIXED...