PROBLEM TO BE SOLVED: To provide a new method for assaying an enzyme involved in the first phase reaction of drug metabolism by PCR, especially establishing a real time detection method, and a probe and a primer pair therefor.西村 益浩...
phase IV,phase IV clinical trial- sometimes the FDA approves a drug for general use but requires the manufacturer to continue to monitor its effects; during this phase the drug may be tried on slightly different patient populations than those studied in earlier trials ...
CYP2D6 genotyping was performed by TaqMan Drug Metabolism Genotyping and Copy Number Assays (Applied Biosystems), using a 7500 Real-time polymerase chain reaction system (Applied Biosystems). The following inactive or reduced function alleles were analyzed: CYP2D6*4 (g.1846G>A; rs3892097), CYP...
Afterwards the labeling reaction was quenched by 3 quick washes with Quenching solution (10 mM sodium ascorbate, 10 mM sodium azide, 5 mM Trolox in PBS). Cells were then fixed for IF analysis or detached from the plates with a cell scraper for WB or MS analysis. Biotin enrichment ...
1; however, an extra participant was enrolled after one participant discontinued the study after their first uneventful dose owing to withdrawal of consent by the participant or guardian. A second participant (cohort 4) discontinued the study after a grade 2 infusion-site reaction (Fig.2)...
The acute phase response is part of the initial reaction to infection and involves secretion of several proteins from the liver, includingCRP,serum amyloid A, andfibrinogen. The release of theseacute phase proteins(part of the non-specific innate immune system) is initiated by cytokines (primarily...
(Leu432Val; rs1056836), COMT (Val158Met; rs4680), CYP17A1 (A1> A2; rs743572); CYP19A1 (C> T; rs10046) and CYP19A1 ((TTTA)n repeats) polymorphisms and BRCA1 founder mutation using TaqMan allelic discrimination method and polymerase chain reaction based restriction fragment length...
If a patient’s body temperature was > 37.5 °C, injection of study treatment was to be postponed until the temperature was < 37.1 °C. Study treatment was to be discontinued in case of an anaphylactic reaction to administration, any AE Grade > 2 as defined by U.S. ...
In detail, G6PD activity was determined as described in the Glucose- 6-Phosphate Dehydrogenase activity assay kit (GMS70013.1 v.A); cell lysates were added to the reaction buffer containing Reagents C&D. After incubation at 25 °C for 2 min, Reagent E was mixed with the reaction mixture,...
Cellular kinetics were evaluated via quantitative polymerase chain reaction (qPCR), and results were analyzed for associations with response and DOR in patients with matched day 14 samples in CD3+ cells sorted by flow cytometry from PB and in whole bone marrow aspirate. sBCMA was measured in ...