Cell colonies containing the construct of interest were selected for growth on agar LB plates supplemented with ampicillin and chloramphenicol. Cells were grown at 37 °C in LB media. Induction of expression was at 30 °C for 8 hours upon the addition of 0.5 M isopropyl β-D-...
Discussion Comparing closed vs. adsorbing boundary conditions, we had previously shown that the effect of a nonreflect- ing boundary (pierced microtiter wells with a water flow underneath) was equivalent to that of a 100-fold lower producer cell density18. In the present report, in order to ...
Stainless steel and insulated P-clips plus reinforcing metal plates were used together with longer screws securing the two aft solar panels to allow the sea cable to be held down along the edge of the solar panels without shading the panels; The center pick point on the mast plate was ultima...
After several gorgeous days, I’m looking at a deluge outside. Good day to curl up with a drama, I guess. Speaking of which, now that WAML is over, I’m down to just 2, AHYI and YTBLSS. I’ve watched the first episodes of a few things but nothing has grabbed me so far. I ...
I came home very late last night, feeling dog-tired: “Help, honey! I can’t get up!” But DDSSLLS should pick my spirits up shortly. credit: deokmis’ tumblr Sometimes, we all need a bit of Jun in our lives…. *** A couple of housekeeping reminders...
I was walked through the Museum by a clerk who would open lights of each hall we went to and closed the lights for the last one, he was pacing our walk and the ‘tour’ lasted barely 5 minutes. There were statues on the floor, in the verandah, on the lawn, everywhere, none were ...
Bacterial growth on the plates were classified as no growth, sparse (n < 20), moderate (20 to 100), or profuse (> 100) on the basis of the number of colonies on the agar plates. Growth of β-haemolytic streptococci, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae ...
Analysis of failed surgery for patellar instability in children with open growth plates. Knee Surg Sports Traumatol Arthrosc. 2012;20: 822-828.Nelitz M, Theile M, Dornacher D, Wolfle J, Reichel H, Lippacher S (2012) Analysis of failed surgery for patellar instability in children with open...
Both the axial and the lateral growth rate and threshold deposition (pyrolysis) temperature of the carbon film deposited on a quartz plate were measured. Knowledge gained from deposition on fused quartz plates was used to determine the deposition conditions on optical fibers. The experimental results...
Translational plate boundaries, which are locations where plates slide parallel to each other, may develop extensional troughs known as pull-apart basins, e.g., the Salton Trough of Southern California [7, p. 131]. Volcanism associated with the Salton Trough generated the heat in the Salton ...