Cell pellets were resuspended and transferred to 96 deep-well plates for DNA extraction using the IBI Scientific 96-well Genomic DNA Bacteria Kit (IBI Scientific: IB47295) per the manufacturer’s protocol. DNA quantification was done using Qubit HS DNA assay, on a Qubit3 device. After DNA qu...
Devil rays are increasingly threatened by target and incidental capture in a wide range of fisheries, from small-scale artisanal to industrial trawl and purse seine fisheries targeting pelagic fishes3,4. The meat is sold or consumed domestically, and the gill plates are exported, mostly to China...
For the cell viability assays, the cells were seeded in 96-well plates at a density of 5000 cells/well (100 μL total volume/well) and were grown for 24 h. The cells were then washed with sterile phosphate-buffered saline and maintained in low-glucose (5.56 mM) DMEM supplemented with ...
Rhizobial strains were grown on plates for 2 days and a liquid culture was grown overnight. The day after transferring germinated seedlings to perlite pots, liquid cultures of rhizobia were spun at 2880 g for 7 min, washed with sterile water 3 times and resuspended in sterile water to an ...
This simple preparation procedure has been widely accepted in various medical fields and spread worldwide. Choukroun, a developer of PRF, further modified it to an advanced form (A-PRF), which is expected to contain a relatively greater number of white blood cells (WBC) [3]. Because of low...
Afterwards, 0.1 ml of the suspensions were aliquoted onto three replicate plates per dilution of agar-nutrient (Acumedia) media for bacteria (pH 7.0) and Martin agar media for fungi (pH 5.0). Cultures were kept at 24 °C to isolate mesophilic microorganisms and at 45 °C for thermophilic ...
3). The compartments are separated by 0.28 m tall bulkheads of steel-supported PVC plates, installed at a 90 ° angle to the bottom and at a distance of 50 cm (Fig. 3c). Bulkheads reduce horizontal flow and function as support for the MR tubes. To allow for release of water over ...
To confer the slow release of BVCs, the bacteria were cultured on medium in stacked plates with covered lids. B. subtilis GB03, which was shown to release volatile compounds that promote plant growth and immunity through in vitro testing, was used as the bacterial strain in this experiment....
Article Open access 04 October 2021 Introduction After landing on the stigma of a flower, a pollen grain germinates and rapidly grows a pollen tube through the style to deliver sperm to the ovule. The rapid polarized growth of the pollen tube involves functional coordination across multiple orga...
using 12-well plates58. Note that after the addition of ferrozine the pH was adjusted to the same pH = 5.7 as the control medium +Fe. However, no change in the pH was detected in agar adjusted with MES as described earlier and in Gruber et al.58. For flg22 treatment, Seeds ...