Cell pellets were resuspended and transferred to 96 deep-well plates for DNA extraction using the IBI Scientific 96-well Genomic DNA Bacteria Kit (IBI Scientific: IB47295) per the manufacturer’s protocol. DNA quantification was done using Qubit HS DNA assay, on a Qubit3 device. After DNA qu...
Tibial dyschondroplasia (TD) is the most-prevalent leg disorder in fast-growing chickens; it is intractable and characterized by abnormal endochondral bone formation of proximal tibial growth-plates (TGPs). Previous studies have shown that bone is a highly vascularized tissue dependent on the coordi...
The rise of growth product management has come with the popularity of product-led growth: as more companies pursue it, they realize that product managers have enough on their plates and shouldn’t have to be responsible for driving both product development and product demand. Laura Wong Former ...
genes brought about a slight increase in average colony size on plates containing 100 μg ml-1kanamycin. This effect was more pronounced in 10851/03 over-expressingporM2than in the strain over-expressingporM1. In DSM 46621 the porin over-expression had an adverse effect on growth upon plating...
Plates were kept at 28°C for 3 to 4 days. The expression of SOS3 or SCaBP8 with an HA-tag in yeast was detected by immunoblot analysis with an anti-HA antibody. Reconstitution of SOS Pathway in Yeast JP837 To confirm the regulation of SOS2 by BIN2, we reconstituted SOS pathway in...
Afterwards, 0.1 ml of the suspensions were aliquoted onto three replicate plates per dilution of agar-nutrient (Acumedia) media for bacteria (pH 7.0) and Martin agar media for fungi (pH 5.0). Cultures were kept at 24 °C to isolate mesophilic microorganisms and at 45 °C for thermophilic ...
Phosphate-solubilizing bacteria (PSB) can enhance plant growth and phosphorus (P) solubilization, it also has been reported to reduce the negative effects of overused agricultural fertilizer in farmland and protect the soil environment. However, the mech
Bidirectional root–shoot signalling is probably key in orchestrating stress responses and ensuring plant survival. Here, we show that Arabidopsis thaliana responses to microbial root commensals and light are interconnected along a microbiota–root–shoo
FoxA2+col.10- (green) cells, FoxA2+col.10+(yellow) cells, or PTHrP+ cells were seeded at a density of 103cells/10 cm culture dishes or 5–10 cells/well into 96-well plates. They were incubated for 21 days in a complete growth medium, with media change every 4–5 days. ...
Open AccessArticle by Weiwei Huang 1,2,3,*, Yongyan Ding 2,4, Shucong Wang 2, Chao Song 5and Fusheng Wang 1,2 1 Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China ...