Two cysteine residues near the N-terminus of GAP-43 are subject to palmitoylation, and are necessary for membrane binding as well as for G(o) activation. N-terminal peptides, which include these cysteines, stimulate G(o). Monopalmitoylation reduces, and dipalmitoylation abolishes the activity ...
This value is not only too large to be the result of the palmitoylation of newly synthesized PLP molecules, it was also unchanged upon the inhibition of protein synthesis with cycloheximide. O enrichment in less actively myelinating 60-day-old rats was significantly reduced. In conclusion, our ...
A minimal code within Gαo N-terminus governs its compartmentalization and re-coding produces G protein versions with shifted localization. We establish the S-palmitoylation at the outer nuclear membrane assay (“SwissKASH”) to probe substrate specificity of PATs in intact cells. With this assay,...
The palmitoylation status of the G-protein G(o)1 alpha regulates its activity of interaction with the plasma membrane.Oxidation catalysts (formation of ... MA Grassie,JF Mccallum,F Guzzi,... - 《Biochemical Journal》 被引量: 88发表: 1994年 ...