Palmitoylation ALK-16 click chemistry kit for WB/MS|全蛋白棕榈酰化ALK-16点击化学试剂盒COA | 说明书规格 价格 期货 询单数量 4 Rxns ¥1408 现货 20 Rxns ¥5960 现货 询单 定制服务 请提交您的留言,我们将为您提供完善的售前支持以满足您的需求。您也可以直接发送电子邮件到project@aimsmass.cn询问。
One reason for this lack of development is the dearth of assays to efficiently screen for small molecular inhibitors of palmitoylation. To address this shortcoming, we have developed a robust, high-throughput compatible, click chemistry-based approach to identify small molecules that interfere with ...
(2014) Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation. Anal Biochem 451, 25-27 CrossRef PubMedB. Ebersole, J. Petko, and R. Levenson, "Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-...
proteins Alkynyl palmitic acid (15-hexadecynoic acid or Palmitic acid (15-yne)) is a modified palmitic acid with an ω-terminal alkyne. Alkynyl palmitic acid often used to identify and characterize the post-translational S-palmitoylation of proteins using the powerful click chemistry (see ...
for palmitoylation, a post-translational protein modification in which a protein acyltransferase transfers the palmitoyl group to a free thiol on target proteins.{26137} Palmitoyl alkyne-CoA is an ω-alkynylated form of palmitoyl CoA. The terminal alkyne group allows the use of click chemistry ...
凭借强大的 click 化学技术(一种简单可靠的两步标记和检测技术),使用 Click-iT® 棕榈酸叠氮化物鉴定和表征棕榈酰化的蛋白质。在第一步中,使用含有叠氮基的生物分子饲养细胞或动物,其主动掺入蛋白质内。与其他标记(例如生物素或荧光染料了解更多信息
A palmitoylation code controls PI4KIIIα complex formation and PI(4,5)P2 homeostasis at the plasma membrane bioRxiv, This content is a preprint and has not been peer-reviewed. 9. Feng, S., et al. (2021). Combining Metabolic Alkyne Labeling and Click Chemistry for Secretome Analysis of ...
The protocol below describes the specific steps for using BMDMs. However, we have also used this protocol in human THP-1, mouse Raw264.7 cells and mouse embryonic fibroblasts. What’s more, we have also used this protocol for detecting protein palmitoylation or succination in BMDMs via other ...
Palladium-triggered deprotection chemistry for protein activation in living cells. Nat. Chem. 6, 352–361 (2014). 33. Martin, B. R. & Cravatt, B. F. Large-scale profiling of protein palmitoylation in mammalian cells. Nat. Methods 6, 135–138 (2009). 34. Lang, K. et al. Genetically...
N. A Decade of Click Chemistry in Protein Palmitoylation: Impact on Discovery and New Biology. Cell Chem. Biol. 2018, 25, 236–246. https://doi.org/10.1016/j.chembiol.2017.12.002.Search in Google Scholar PubMed 106. Best, M. D. Click Chemistry and Bioorthogonal Reactions: Unprecedented...