Autopalmitoylation of tubulin. Protein Sci 9, 1357-1364 (2000).Wolff J, Zambito AM, Britto PJ, Knipling L. 2000. Autop- almitoylation of tubulin. Protein Sci 9:1357A1364.Wolff, J., Zambito, A.M., Britto, P.J., and Knipling, L. 2000. Auto- palmitoylation of tubulin. Protein ...
Using activity-based chemical probes, we discovered that human TEADs possess intrinsic palmitoylating enzyme-like activities and undergo autopalmitoylation at evolutionarily conserved cysteine residues under physiological conditions. We determined the crystal structures of lipid-bound TEADs and found that the ...
Autopalmitoylation of TEAD proteins regulates transcriptional output of the hippo pathway. Nat Chem Biol. 2016;12:282-9.Chan, P. et al. Autopalmitoylation of TEAD proteins regulates transcriptional output of the Hippo pathway. Nat. Chem. Biol. 12, 282-289 (2016)....
Polymerization to microtubules, or use of tubulin S, markedly decreases the accessibility of the palmitoylation sites. Palmitoylation increases the electrophoretic mobility of a portion of alpha tubulin toward the beta band. Palmitoylated tubulin binds a colchicine analogue normally, but during three warm...
Protein S-palmitoylation attaches fatty acid (palmitate) to cysteine residues, and regulates protein trafficking, membrane localization and signaling activities. Using activity-based chemical probes, we discovered that human TEADs possess intrinsic palmitoylating enzyme-like activities, and undergo auto...
autopalmitoylation at evolutionarily conserved cysteine residues under physiological conditions. We determined the crystal structures of lipid-bound TEADs and found that the lipid chain of palmitate inserts into a conserved deep hydrophobic pocket. Strikingly, palmitoylation did not alter TEAD's localization...
Our lab has developed an assay amenable to high throughput screening, which measures the rate of the first step of the palmitoylation reaction, autopalmitoylation. During autopalmitoylation there is a release of CoASH, which is then coupled to an alpha-ketoglutarate dehydrogenase reaction to produce ...
Palmitoylation refers to the covalent attachment of fatty acids, such as palmitate, onto the cysteine residues of proteins. This process may subsequently alter their localization and function. Nearly all of the enzymes that catalyze palmitoylation, zDHHC protein acyl transferases (PATs), are implicated...
TEAD TRANSCRIPTION FACTOR AUTOPALMITOYLATION INHIB 专利名称：TEAD TRANSCRIPTION FACTOR AUTOPALMITOYLATION INHIBITORS 发明人：WU, Xu 申请号：US2016/053318 申请日：20160923 公开号：WO2017/053706A1 公开日：20170330 专利内容由知识产权出版社提供 专利附图：摘要：The present disclosure compounds, as well as ...
Wolff J dg8.niddk.nih.gov,Zambito AM,Britto PJ,Knipling L. Autopalmitoylation of tubulin.[J].Protein Science: A Publication of the Protein Society 2000,7(7).Wolff J, Zambito AM, Britto PJ, Knipling L. Autopalmitoylation of tubulin. Protein Sci. 2000; 9 :1357–1364....