This ability of OFUT1 to facilitate folding of Notch did not require its fucosyltransferase activity. Thus, a glycosyltransferase can bind its substrate in the endoplasmic reticulum to facilitate normal folding. 展开 关键词: Chaperone Activity of Protein O-Fucosyltransferase 1 Promotes Notch Receptor ...
O-Fucosylation is mediated by protein O-fucosyltransferase 1, and down-regulation of this enzyme by RNA interference or mutation of the Ofut1 gene in Drosophila or by mutation of the Pofut1 gene in mouse prevents Notch signaling. To investigate the molecular basis for the requirement for O-...
In the crystal structure, two SPY molecules interact with each other in a head-to-tail manner, forming a symmetrical dimer with a length of ~150 Å, a width of ~70 Å and a height of ~75 Å (Fig.1c). The dimer gives rise to two distinct interfaces: interface 1 (and ...
The wbsJ gene from Escherichia coli O128:B12 encodes an al,2-fucosyltransferase responsible for adding a fucose onto the galactose residue of the 0-antigen repeating unit via an α1,2 linkage. The wbsJ gene was overexpressed in E. coli BL21 (DE3) as a fusion protein with glutathione S-tra...
Using the GST-WbiQ fusion protein, the wbiQ gene was identified to encode an α1,2-fucosyltransferase using a radioactivity based assay, thin-layer chromatography assay, as well confirming product formation by using mass spectrometry and NMR spectroscopy. The fused enzyme (GST-WbiQ) has an ...
The wbsJ gene from Escherichia coli O128:B12 encodes an α1,2-fucosyltransferase responsible for adding a fucose onto the galactose residue of the O-antigen repeating unit via an α1,2 linkage. The wbsJ gene was overexpressed in E. coli BL21 (DE3) as a fusion protein with glutathione S-...
In industrialized countries, the frequency of these organisms has decreased, but they continue to be an important cause of diarrhea [1]. EPEC are divided into typical and atypical subgroups based on the presence of the adherence factor (EAF) plasmid [2]. The most studied EPEC strains belong ...