281, 9385-9392), we demonstrated that an enzyme distinct from protein O-fucosyltransferase 1 adds O-fucose to TSRs. A known homologue of O-fucosyltransferase 1 is putative protein O-fucosyltransferase 2. The cDNA sequence encoding O-fucosyltransferase 2 was originally identified during a data base...
Protein O-fucosyltransferase 2 (POFUT2) initiates metazoan TSR O-glycosylation and plays an important role in the folding and stabilization of these proteins (1213, 14, 1516). Recently, TSR O-glycosylation has also been observed in Plasmodium falciparum and Plasmodium vivax (17, 18); this O-...
The overall structure ofArabidopsisSPY monomer shows little similarity with that of human ER-localized protein O-fucosyltransferase POFUT1/233,34but most resembles that of human OGT35,36(Supplementary Fig.5). The catalytic regions inArabidopsisSPY and human OGT can be superimposed well with a RMSD...
This ability of OFUT1 to facilitate folding of Notch did not require its fucosyltransferase activity. Thus, a glycosyltransferase can bind its substrate in the endoplasmic reticulum to facilitate normal folding. 展开 关键词: Chaperone Activity of Protein O-Fucosyltransferase 1 Promotes Notch Receptor ...
(Fig.4). ProteinO-fucosyltransferase 1 (POFUT1) addsO-fucose (Fuc) to Ser/Thr residues within the C2–C3(C2X4-5(S/T)C3) of EGF repeats38,39,40, which is further extended by a Fringe enzyme required for Notch-ligand binding17,41. AnO-GlcNAc modification is also added to Ser/Thr...
are superposed to illustrate how the ligandability scores recapitulate the observed binding site. These are protein representative chains and ligand molecules might not be observed in the same entry.AGDP-fucose proteinO-fucosyltransferase 2, Q9Y2G5, with GDP-L-fucose (GFB) superimposed (PDB: 4AP6...
(EGF) repeat domains, Serine and Threonine residues of consensus sequences are subjects for O-linked fucosylation (Holdener and Haltiwanger2019). The enzyme Protein O-fucosyltransferase-2 (POFUT2) adds O-fucose to TSP1 in the endoplasmic reticulum, to the consensus sequence C-X-X-S/T-C, ...
Notably, Protein O-fucosyltransferase 1 (Ofut1/Pofut1), a soluble, ER localized enzyme that fucosylates Epidermal Growth Factor-like (EGF) repeats, functions as a chaperone involved in the proper localization of the Notch receptor in certain contexts. Pofut2, a related enzyme that modifies ...
Its molecular basis has been identified as a defect in the transporter of GDP-L-fucose into the Golgi lumen, which reduces the availability of the substrate for fucosyltransferases. During metabolic radiolabeling experiments using [³H]fucose, LAD II fibroblasts incorporated significantly less radio...
(GalNAc-T4), GalNAc-T14, GalNAc-T6, fucosyltransferases 4 (FUT4), FUT8, andO-GlcNAc transferase (OGT) [29,90,91,92], which are overexpressed in breast cancer and correlated with its prognosis, may be used as novel biomarkers or combined with traditional biomarkers to improve the ...